护卵汤配合补佳乐及安宫黄体酮对卵巢早衰模型小鼠卵巢Th17和Treg的影响

目的观察护卵汤配合补佳乐及安宫黄体酮对卵巢早衰(POF)模型小鼠卵巢Th17和Treg表达水平的影响。方法采用透明带多肽(PZP3)及弗氏完全佐剂(CFA)和弗氏不完全佐剂(IFA)建立POF模型小鼠,随机分为空白组、模型组、护卵汤+补佳乐+安宫黄体酮组(护补安组)、补佳乐+安宫黄体酮组(补安组)、护卵汤组,护卵汤分别给药对POF模型小鼠进行干预后,采用酶联免疫吸附(ELISA)法检测各组小鼠的血清促卵泡生成素(FSH)、雌二醇(E2)含量;逆转录-聚合酶链反应(RT-PCR)检测小鼠卵巢的Th17和Treg表达水平;光镜下观察各组卵巢组织形态学变化。结果与模型组比较,护补安组、补安组、护卵汤组对致衰小鼠有治疗作用,差异具有统计学意义(P<0.01);护补安组、补安组在降低小鼠血清FSH含量方面优于护卵汤组(P<0.05);但护卵汤组在降低小鼠卵巢Th17水平上优于补安组,差异具有统计学意义(P<0.05)。结论护卵汤配合补佳乐及安宫黄体酮可通过降低小鼠卵巢Th17而升高小鼠卵巢Treg水平治疗卵巢早衰模型小鼠,且在降低小鼠血FSH含量上优于单纯护卵汤组。

Effects of Huluantang combined with progynova and medroxyprogesterone acetate on the expressions of Th17 and Treg in premature ovarian failure mice model

Objective To observe the effects of Huluantang combined with progynova and medroxyprogesterone acetate on the expressions of Th17 and Treg of ovarian in premature ovarian failure(POF) mice.Methods The mouse model of autoimmune POF was established by injecting the zona pellucida 3 peptide fragments(pZP3) combined with complete Freund adjuvant(CFA) and incomplete Freund adjuvant(IFA).After immunization,the mice were randomly divided into 5 groups as follows: blank group,modeling group,progynova combined with medroxyprogesterone group,Huluantang group and Huluantang combined with progynova and medroxyprogesterone acetate group.After treatment,follicle stimulating hormone(FSH) and estradiol(E2) in serum were detected by ELISA,the Th17 and Treg expression levels in ovarian were examined by RT-PCR,the ovarian morphology changes were observed under light microscope.Results Compared with modeling group,there were therapeutic effects on treating POF mice in Huluantang combined with progynova and medroxyprogesterone acetate group,progynova combined with medroxyprogesterone acetate group and Huluantang group,the differences were statistically significant(all P<0.01).In terms of reducing serum FSH,Huluantang combined with progynova and medroxyprogesterone acetate group and progynova combined with medroxyprogesterone group were superior to Huluantang group(all P<0.05).But in terms of reducing Th17 levels of ovarian,Huluantang group was superior to progynova combined with medroxyprogesterone group(P<0.05).Conclusion It is effective to treat autoimmune POF mice with Huluantang combined with progynova and medroxyprogesterone acetate which may be related with reducing the Th17 levels and increasing the Treg levels.And it is better than traditional chinese medicine group in reducing mice serum FSH levels.