Sequence Analysis of the VP4, VP6, VP7, and NSP4 Gene Products ofthe Bovine Rotavirus WC3 |
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Authors: | Ciarlet Max Hyser Joseph M Estes Mary K |
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Institution: | (1) Department of Molecular Virology and Microbiology, Bayor College of Medicine, One Baylor Plaza, Mailstop BCM-385, Houston, TX 77030, USA;(2) Present address: Bioprocess and Bioanalytical Research, Merck Research Laboratories, West Point, PA, 19486, USA;(3) Department of Molecular Virology and Microbiology, Bayor College of Medicine, Houston, TX 77030, USA |
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Abstract: | The bovine rotavirus (BRV) WC3 serves as the background strain in the development of a multivalent reassortant vaccine against rotavirus gastroenteritis in infants. The genes encoding the outer capsid spike protein VP4, the inner capsid protein VP6, the outer capsid glycoprotein VP7, and the viral enterotoxin NSP4 of BRV WC3 were sequenced. Comparative analysis of the deduced amino acids of the sequenced genes indicated that the BRV WC3 strain shares a high degree of amino acid identity with serotype P75] VP4 (93–96%), serotype G6 VP7 (91–97%), subgroup (SG) I VP6 (96–99%), and NSP4 genogroup A (96–98%) BRV strains. Our results confirm and extend previous studies which suggested that the VP4 of BRV WC3 was closely related to that of the P75] prototype, BRV UK. In addition, the VP6 and VP7 of BRV WC3 were very similar to the VP6 and VP7 of both SG I and G6 BRV NCDV and UK strains. However, the NSP4 of BRV WC3 was more closely related to that BRV NCDV, the P61] prototype, than to that of BRV UK. |
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Keywords: | rotavirus vaccine VP4 VP6 VP7 NSP4 WC3 |
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