LH750血细胞分析仪复检规则制定及应用评价

目的采用LH750血细胞分析仪评估国际血液学复检专家组推荐的血细胞复检规则，通过对实验数据进行分析建立适合于该仪器的血细胞复检规则。方法采用LH750五分类全自动血细胞分析仪检测患者标本2360份．同时涂片做显微镜检查，包括人工白细胞分类和细胞形态观察，按照国际血细胞复检规则和涂片镜检阳性规则进行评估，计算出真阳性、真阴性、假阳性和假阴性的比率。通过分析假阳性及假阴性的原因，并结合医院实际情况和仪器的性能特点制定出适合于该仪器的血细胞复检规则。结果根据国家血液学复检专家组推荐的41条复检规则和涂片镜检阳性规则对检测结果进行统计分析，真阳性率为9．1％，假阳性率为28．1％，真阴性率为62．2％，假阴性率为0．55％复检率为37．2o．4；应用修改后规则进行统计分析，真阳性率为8．7％，假阳性率为11．6％，真阴性率为79．1％，假阴性率为1．2％，复检率为20．3％。采用前后两个规则均未漏检原幼细胞，复检率明显降低，假阴性率略有升高。结论国际血细胞复检规则假阳性率较高，实验室应根据医院情况和仪器特点制定适宜的血细胞分析复检规则，从而提高工作效率，保证血细胞分析结果的准确性。

LH750 blood cell analyzer inspection rules and application evaluation

Objective To adopt LH750 blood cell analyzer to evaluate international hematology review panel recom- mended blood cells review criteria, based on the analysis of experimental data to establish suitable for the apparatus of blood cells review rules. Methods using the LH750 five classification automatic blood cell analyzer with 2 360 specimens, and microscopic examination of smears do, including manual white blood cell classification and morpho- logical observation on cells, in accordance with international blood smear review criteria for rules and smear positive rules are evaluated, calculate the true positive, true negative, false positive and false negative rate. Through the a- nalysis of false positive and false negative cause, and combine the actual situation of the hospital and the instrument performance characteristics of the development of a suitable for the apparatus of blood cells review rules. Results according to the state of Hematology review panel recommended 41 review criteria and smear positive rules on the test results of statistical analysis, the true positive rate is 9.1%, false positive rate is 28.1%, true negative rate was 62.2 %, the false negative rate was 0.55 %, reinspection rate was 37.2 %, Application of the modified rules for statis- tical analysis, the true positive rate was8.7%, false positive rate wasll. 6%, true negative rate was79.1%, false negative rate wasl. 2%, for reinspeetion rate was20.3%. Before and after the use of the two rules were not missed the original young cell review rate was significantly reduced, the false negative rate increased slightly. Conclusions international blood smear review criteria rules the false positive rate is higher, the laboratory shall according to the hospital and equipment characteristics of the blood cell analysis review criteria, which can improve the work efficien- cy, guarantee the accuracy of the results of blood cell analysis.