| 背部循经推拿手法对亚健康模型大鼠海马神经元凋亡相关基因Bcl-2、Bax及Casepase-3表达的影响 |
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| 引用本文： | 张海英,雷龙鸣,邱石源,唐宏亮,庞军,吴华裕,王开龙,伦轼芳.背部循经推拿手法对亚健康模型大鼠海马神经元凋亡相关基因Bcl-2、Bax及Casepase-3表达的影响[J].中华中医药学刊,2019(3):579-582,I0002. |
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| 作者姓名： | 张海英 雷龙鸣 邱石源 唐宏亮 庞军 吴华裕 王开龙 伦轼芳 |
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| 作者单位： | 1.广西中医药大学;2.广西中医药大学第一附属医院推拿科;3.右江民族医学院附属医院;4.广西中医药大学第一附属医院中医治未病中心 |
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| 基金项目： | 国家自然科学基金项目(81460747;81160457) |
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| 摘 要： | 目的:观察背部循经推拿对亚健康模型大鼠海马神经元凋亡相关基因Bcl-2、Bax及Casepase-3蛋白表达的影响。方法:60只wistar大鼠随机分为正常组、模型组和推拿干预组,后两组予束缚应激,推拿干预组予背部循经推拿。实验结束后,采用免疫组化法检测3组大鼠海马组织Bcl-2、Bax及Casepase-3蛋白表达,实时荧光定量PCR方法检测Casepase-3 mRNA的表达情况。结果:模型组大鼠海马组织Bcl-2蛋白表达下降、Bax与Caspase-3蛋白表达上升,与空白组比较,差异有显著性(P<0.05);推拿干预组海马组织Bcl-2蛋白表达上调、Bax蛋白表达下调、Caspase-3蛋白及Caspase-3 mRNA表达均上升,与模型组比较,差异有显著性(P<0.05)。结论:背部循经推拿手法可以通过抑制Bax与Caspase-3蛋白及Caspase-3 mRNA表达、上调Bcl-2蛋白表达,从而发挥对慢性应激所致亚健康状态的脑神经保护作用。
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| 关 键 词： | 亚健康 应激 推拿 海马神经元 |
Effects of Chinese Tuina along Back Meridians on Expressions of Apoptosis-Related Genes Bcl-2, Bax and Casepase-3 in Hippocampal Neurons of a Subhealth Rat Model |
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| ZHANG Haiying,LEI Longming,QIU Shiyuan,TANG Hongliang,PANG Jun,WU Huayu,WANG Kailong,LUN Shifang.Effects of Chinese Tuina along Back Meridians on Expressions of Apoptosis-Related Genes Bcl-2, Bax and Casepase-3 in Hippocampal Neurons of a Subhealth Rat Model[J].Chinese Archives of Traditional Chinese Medicine,2019(3):579-582,I0002. |
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| Authors: | ZHANG Haiying LEI Longming QIU Shiyuan TANG Hongliang PANG Jun WU Huayu WANG Kailong LUN Shifang |
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| Institution: | (Guangxi University of Traditional Chinese Medicine, Nanning 530200 , Guangxi, China;Tuina Department of Die First Affiliated Hospital to Guangxi University of Traditional Chinese Medicine,Nanning 530023,Guangxi,China;The Affiliated Hospital of Youjiang Medical University for Nationalities, Nanning 533000 ,Guangxi,China;The Traditional Chinese Medicine and Disease Prevention Center of The First Affiliated Hospital toGuangxi University of Traditional Chinese Medicine, Nanning 530023 , Guangxi, China;Department of Cell Biology and Genetics, Guangxi Medical University,Nanning 530021 ,Guangxi,China;The Rehabilitation Department of The First Affiliated Hospital to Guangxi University ofTraditional Chinese Medicine, Nanning 530023 , Guangxi, China;Guangxi Yuan Health Industry Co. ,Ltd. ,Nanning 530022 ,Guangxi, China) |
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| Abstract: | ZHANG Haiying;LEI Longming;QIU Shiyuan;TANG Hongliang;PANG Jun;WU Huayu;WANG Kailong;LUN Shifang(Guangxi University of Traditional Chinese Medicine, Nanning 530200 , Guangxi, China;Tuina Department of Die First Affiliated Hospital to Guangxi University of Traditional Chinese Medicine,Nanning 530023,Guangxi,China;The Affiliated Hospital of Youjiang Medical University for Nationalities, Nanning 533000 ,Guangxi,China;The Traditional Chinese Medicine and Disease Prevention Center of The First Affiliated Hospital toGuangxi University of Traditional Chinese Medicine, Nanning 530023 , Guangxi, China;Department of Cell Biology and Genetics, Guangxi Medical University,Nanning 530021 ,Guangxi,China;The Rehabilitation Department of The First Affiliated Hospital to Guangxi University ofTraditional Chinese Medicine, Nanning 530023 , Guangxi, China;Guangxi Yuan Health Industry Co. ,Ltd. ,Nanning 530022 ,Guangxi, China) |
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| Keywords: | sub-health stress Tuina hippocampal neurons |
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