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Detection and Identification of Single Molecules in Immunology and Immunohematology: Single (solution)-phase Single-molecule FCS
Authors:Ulrike Demel  Zeno Földes-Papp
Affiliation:Clinical Immunology and Jean Dausset Laboratory, Graz University M.S. and Hospital, Auenbruggerplatz 8, A-8036 Graz, LKH, Austria
Abstract:In the Goodpasture experiment, we determined a probability of 99.1% of identifying one single immune complex. Under these conditions, a single molecule event is proven. There exist no instrumental assumptions of our approach on which the experiments themselves, the theoretical background or the conclusion are based on. Our results open up a broad field for analytics and diagnostics in solution, particularly in immunology and immunohematology.
Keywords:Poisson distribution  Critical bulk concentration  Fluorescence correlation spectroscopy  Solution-phase single-molecule fluorescence correlation spectroscopy  Single immune complex  Goodpasture autoantibodies
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