丹参注射液给药时间对预防顺铂耳毒性作用的影响

目的探索丹参注射液给药时间对预防顺铂耳毒性作用的影响。方法健康杂色豚鼠40只。随机均分为4组。1．对照组：腹腔注射顺铂1mg／kg／d。2．实验Ⅰ组：腹腔注射顺铂1mg／kg／d，6h后腹腔注射丹参注射液8mL／kg／d。3．实验Ⅱ组：腹腔同时注射顺铂和丹参注射液，剂量同上。4．空白组：腹腔注射生理盐水0．8mL／kg／d。各组连续6d。各组动物在实验前及结束后各做一次BAEP检测。以ABRⅢ波反应阈为检测听阈。结果对照组与实验组听阈在实验前后同组内相比均有提高，分别为：42．6±4．32dB、33±3dB、36．6±2．16dB，空白组为31．5±2．25dB。但实验Ⅰ组和实验Ⅱ组听阈分别比对照组低，差别显著。实验前后同组内相比听阈增加率：实验Ⅰ组〈实验Ⅱ组〈对照组，分别为：（6±8．4）％、（19．67±4．53）％、（39±17．4）％，差别显著。实验Ⅰ组与空白组相比无显著性差别。结论丹参注射液预防顺铂耳毒性作用与给药时间有关；认为丹参在顺铂使用后6h给药其预防顺铂耳毒性的作用更为显著。

Effects of Administration Time of Salvia Miltiorrhiza Injection on Precaution the Ototoxicity of Cisplatin

Objective To explore the effects of Administration time of Salvia miltiorrhiza injection on precaution the ototoxicity of Cisplatin.Methods 40 guinea pigs were randomly divided into 4 groups： control group, experiment group Ⅰ, experiment group Ⅱ and empty group. Control group were injected Cisplatin 1 mg/kg/d by intraperitoneal. Eexperiment group Ⅰ ,6 hours after injection ofCisplatin1mg/kg/d by intrapefitoneal,were injected Salvia miltiorrhiza injection 8mL/kg/d by intraperitoneal. Experiment group Ⅱ were injected Cisplatin 1 mg/kg/d and Salvia miltiorrhiza injection 8mL/kg/d by intraperitoneal simultaneously.And empty group were injected normal sodium 0.8mL/kg/d by intraperitoneal. One time everyday, 6 days in all. Before and after the injection,BEAP was detected,and reaction threshold of ABR Ⅲ was taken as detected audibility threshold（dB）.Results Audibility thresholds from control group,experiment group Ⅰ and experiment group Ⅱ were all rised,and they were （42.6 ± 4.32）dB,（33.0 ± 3.00） dB and （36.6 ± 2.16）dB respectively.Audibility thresholds from empty group was （31.5 ± 2.25）dB.Experiment group Ⅰ and experiment group Ⅱ were lower than control group,P〈0.05. The augmented rate： experiment group Ⅰ 〈 experiment group Ⅱ 〈 control group, ang they were （6 ±8.4）%,（19.67 ±4.53）% and （39 ± 17.4）%, P〈 0.05.There was no significant defference between experiment group Ⅰ and empty group.Conclusion It is related with administration time that the effect of Salvia miltiorrhiza injection on precaution the ototoxicity of Cisplatin;and 6 hours after Cisplatin using Salvia miltiorrhiza injection, the effect is more significant.