|
|||||
|
|
| 靶向沉默Notch1基因对膀胱癌细胞系T24增殖的影响 | |
| 引用本文： | 艾星,张秀萍,吴准,刘双林,臧桐,张国晖, 孙凤岭,张水文张勇,张旭.靶向沉默Notch1基因对膀胱癌细胞系T24增殖的影响[J].山东大学学报(医学版),2009,47(9):53-57. |
| 作者姓名： | 艾星 张秀萍 吴准 刘双林 臧桐 张国晖 孙凤岭 张水文张勇 张旭 |
| 作者单位： | 1. 中国人民解放军北京军区总医院泌尿外科， 北京 100700； 2. 山东大学附属济南市中心医院医学实验诊断中心， 济南 250013； 3. 华中科技大学同济医学院附属同济医院泌尿外科， 武汉 430030； 4. 中国人民解放军总医院泌尿外科， 北京 100086 |
| 基金项目： | 国家自然科学基金资助课题（30571858）。 |
| 摘 要： | 目的探讨Notch1基因沉默对人膀胱癌细胞增殖的影响。方法用pGenesil质粒构建靶向Notch1的siRNA真核表达载体psiRNA1，并将其转染到膀胱癌细胞系T24中，四甲基偶氮唑盐微量酶反应比色法(MTT)、流式细胞术(FCM)检测Notch1沉默后膀胱癌细胞生长变化、细胞周期和凋亡情况。逆转录聚合酶链反应（RT PCR）和蛋白印记法（Western Blot）检测Notch1基因的mRNA和蛋白在转染前后表达变化。结果转染psiRNA1质粒后T24细胞的生长受到显著抑制，呈一定的时间依赖性（60?h内），凋亡率增加，G0/G1期细胞明显增多（未转染组、空载体组、阴性对照组），与对照组比较差异有统计学意义（P＜0.05），且Notch1基因在mRNA和蛋白水平表达相对于对照组明显下调（P＜0.05）。结论沉默Notch1基因能够抑制膀胱癌细胞的增殖，Notch1在膀胱癌中可能具有癌基因的作用。 |
| 关 键 词： | 基因，Notch1 T24细胞 膀胱肿瘤 增殖 |
| 收稿时间： | 2009-03-03 |
Effect of silencing Notch1 on proliferation of bladder cancer cell line T24 |
|
| AI Xing,ZHANG Xiu ping,WU Zhun,LIU Shuang lin,ZANG Tong,ZH ANG Guo hui,SUN Feng ling,ZHANG Shui wen,ZHANG Yong,ZHANG Xu.Effect of silencing Notch1 on proliferation of bladder cancer cell line T24[J].Journal of Shandong University:Health Sciences,2009,47(9):53-57. | |
| Authors: | AI Xing ZHANG Xiu ping WU Zhun LIU Shuang lin ZANG Tong ZH ANG Guo hui SUN Feng ling ZHANG Shui wen ZHANG Yong ZHANG Xu |
| Institution: | 1. Department of Urology, General Hospital of Beijing Military Comm and of Chin ese PLA, Beijing 100700; 2. Center of Medical Laboratory Diagnosis, Jinan Centra l Hospital, School of Clinical Medicine, Shandong University, Jinan 250013, China; 3. Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong U niversity of Science and Technology, Wuhan 430030, China; 4. Department of Urology, Chinese PLA General Hospital, Beijing 100086, China |
| Abstract: | To investigate the effect of silencing Notch1 gene on cell proliferation in vitro in bladder cancer cell line T24. MethodsThe siRNA eukaryotic expression vector of Notch1（psiRNA1）was constructed using pGenesil plasmid. psiRNA1 was transfected into bladder cancer cell line T24 by lipofectamine. Cell growth, cycle and apoptosis of T24 were determined with methabenzthiazuron (MTT) and flow cytometry (FCM) assay after transfection. The mRNA and protein expressions of Notch1 were determined by RT PCR and western blot. ResultsCompared with the control group, the growth curve of T24 cells was inhibited after transfection, which was time dependent during 60 hours. Notch1 silencing produced a cell cycle arrest at G0/G1（P＜0.05）. The apoptotic cell index also increased to some degree. mRNA and protein expressions of Notch1 were significantly down regulated compared with the controls after transfection（P＜0.05）. Conclusion Notch1 could inhibit the proliferation of bladder cancer cells by silencing expression of Notch1 , and it might act as a carcinogenic gene in bladder cancer. |
| Keywords: | Gene， Notch1 T24 Bladder neoplasms Proliferation |
| 点击此处可从《山东大学学报(医学版)》浏览原始摘要信息 | |
| 点击此处可从《山东大学学报(医学版)》下载免费的PDF全文 | |