顺铂诱导人结肠癌细胞凋亡的实验研究

 采用细胞培养方法，通过AO染色，DNA微电泳及电镜观察顺铂作用下结肠癌细胞凋亡过程。实验结果表明：结肠癌SW1116细胞在不同浓度的顺铂作用2小时后，呈现不同程度的凋亡。电镜观察到凋亡的细胞表现为细胞核皱缩，核内染色质结块状，趋边缘性分布；DNA微电泳呈拖尾现象，提示凋亡细胞内DNA片断化；凋亡细胞AO染色呈阳性反应，其阳性率随顺铂的浓度增高而递增，与对照组相比，差异有显著意义（P＜0．05）。因此认为顺铂可诱导和促进结肠癌细胞凋亡，这一现象可能是顺铂抗癌作用之一。

Effect of Cisplatin on the Induction of Apoptosis in Colonic Carcinoma Cell Lines

To investigate the effect of cisplatin on the induction of apoptosis in colonic carcinoma. Methods: A 2-hour exposure of SW1116 human colonic carcinoma cell line in index phase of growth to various levels of cisplatin resulted in the apoptosis,which is detected by gel electrophoresis of DNA,stainig with AO and electronic microscopy. Results:The apoptosis had occured in SW1116 was confirmed by electronic microscopy and gel electrophoresis of DNA. Morphologically,apoptotic cell death is characterized by cell shrinkage and aggregation of the chromation into dense,often crescent—shaped masses under the nuclear membrane. The staining with AO and fragmentation of DNA showed that the rate of apoptosis was dependent on the dose of cisplatin. A percentage of the total apoptotic cell number,compared with control group, has significant difference (P＜0.05).Conclusion:Cisplatin may induce and promote the apoptosis of colonic carcinoma.