| 亚硒酸钠和维甲酸联合对HL-60细胞c-myc和c-fos基因表达的影响 |
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| 引用本文： | 刘新光,于树玉.亚硒酸钠和维甲酸联合对HL-60细胞c-myc和c-fos基因表达的影响[J].肿瘤防治研究,1998,25(3):173-177. |
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| 作者姓名： | 刘新光 于树玉 |
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| 作者单位： | 1. 广东医学院医用生化研究所, 湛江 524023;
2. 中国医学科学院肿瘤研究所生化室 |
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| 摘 要： | 以HL-60细胞为实验对象，利用Slot-blot技术，观察了亚硒酸钠和维甲酸（RA）联合对其原癌基因c-myc和c-fos表达的影响。结果表明：硒和RA在第一和第二天都可使c-myc基因表达下降，以后逐渐上升到对照组或稍高水平。联合可使c-myc表达显著下降，强于两单独作用。硒和pA在第三至第四天均可使c-fos表达明显升高，第四天5.8ｕmol/L Na2SeO3 和0.1ｕmol/LRA以及联合组c-fos表达水平分别为对照组的2.8，3.7和5.4倍。
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| 关 键 词： | 亚硒酸钠 维甲酸 药物联合作用 HL－60细胞 c－myc c-fos |
Combined effects of sodium selenite and all-trans retioic acid on c-myc and c-fos oncogenes expression of human pronyelocytic leukemia cells(HI-60) |
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| Liu Xinguang,et al.Combined effects of sodium selenite and all-trans retioic acid on c-myc and c-fos oncogenes expression of human pronyelocytic leukemia cells(HI-60)[J].Cancer Research on Prevention and Treatment,1998,25(3):173-177. |
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| Authors: | Liu Xinguang |
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| Institution: | Institute of Medical Biochemistry, Guangdong Medical College, Zhanjiang 524023 |
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| Abstract: | HL-60 cell line was used to study the combined effects of sodium selenite and all transretinoic acid (RA) on c-myc and c-fos oncogenes expression,Slot blot analysis domonstratedthat 1-2 days after treatment by Na2SeO3 and RA cells showed decreased expression of cmyc, but further treatment slightly increased expression of c-myc gene. Combined treatmentdecreased expression of c-myc more significantly. Both Na2SeO3 and RA showed high expression of c-fos after 3-4 days. 4 days after treatment with 5.8μmol/L Na2SeO3, 0.1μmol/LRA and combined treatment, the expression of c-fos were 2.8, 3.7 and 5.4 times that ofcontrol respectively. |
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| Keywords: | Sodium selenite All-trans retinoic acid Combined effects Human promyelocytic leukemia cell line(HL-60) c-myc c-fos |
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