再生障碍性贫血患者骨髓祖细胞培养的临床意义

目的探讨再生障碍性贫血（AA）患者红细胞集落生成单位（CFU—E）、爆式红细胞集落生成单位（BFU-E）、粒细胞-单核细胞集落生成单位（CFU—GM）及巨核细胞集落生成单位（CFU-Meg）骨髓祖细胞培养情况。方法抽取39例AA患者骨髓分别作四类祖细胞培养，在7d、14d测定其集落数，与同期住院非血液病患者比较。结果39例再障患者四类集落数均值与对照组比较，差异有统计学意义（P〈0．01或P〈0．001）。集落数随着病情加重而逐步下降。治愈及进步者四类集落数接近对照组水平，治疗无效及进展者与治疗前比较差异无统计学意义。结论部分AA患者存在四类祖细胞培养集落数的降低，基本与患者的病情严重程度呈正比。动态监测AA患者骨髓四类祖细胞集落数的变化对辅助诊断、观察疗效及预后判断均有指导意义。

Clinical significance and culture of hematopoietic progenitor cells in aplastic anemia

Objective To study hematopoietie progenitor cells（HPC） proliferation through detection of blast-forming-unit of erythyroeyte （BFU-E）, colony forming-unit of erythroeyte （ CFU-E ）, colony-forming-unit of granuloeyte-monoeyte （ CFU-GM ） and colony-forming-unit of megakayocyte（CFU-Meg） by HPC culture of bone marrow（BM） from aplastie anemia（AA）. Methods Detecting BFU：E,CFU-E, CFU-GM and CFU-Meg at 7 d, 14 d after cellular culture from 39 eases with AA. Results The mean values of four different colonies in 39 patients were significandy lower than those of the control. The mean decrease of these colonies was positively correlated with the severity of the types of diseases.Conclusion A portion of eases with AA have lower quantities of BFU-E, CFU-E, CFU-GM and CFU-Meg, and the quantitative decrease in these color hies is positively correlated with severity of the disease. Testing the changes of BFU-E,CFU-E,CFU-GM and CFU-Meg by HPC culture has significance to the diagnosis, effect observeing and prognosis judging.