首页 | 本学科首页   官方微博 | 高级检索  
检索        

蒿甲醚对小鼠体内日本血吸虫磷酸化酶,乳酸脱氢酶,三磷酸腺苷 …
引用本文:肖树华,尤纪青.蒿甲醚对小鼠体内日本血吸虫磷酸化酶,乳酸脱氢酶,三磷酸腺苷 …[J].中国药理学报,1999,20(8):750-754.
作者姓名:肖树华  尤纪青
作者单位:[1]中国预防医学科学寄生虫病研究所,世界卫生组织疟疾,血吸虫 [2]中国预防医学科学寄生虫病研究所,世界卫生组织疟
摘    要:目的:观察蒿甲醚(Art)对日本血吸早磷酸化酶(PP)、乳酶脱氢酶(LDH)、6-磷酸工唿科(G-6-PDH)和三磷酸腺苷酶(ATPase)的影响。方法:感染32-38天的小鼠于灌服Art100-300mg.kg^-1后24-72h剖 杀,收集雌(♀)、雄虫(0165)、按NADH和NADPH的形成和无机磷的释放量测定 上述4种酶。结果:感染小鼠用Art300mg.kg^-1治疗后24-48h,♀

关 键 词:日本血吸虫  蒿甲醚  磷酸化酶  药理  乳酸脱氢酶

Effect of artemether on phosphorylase, lactate dehydrogenase, adenosine triphosphatase, and glucosephosphate dehydrogenase of Schistosoma japonicum harbored in mice.
S H Xiao,J Q You,H F Guo,J Y Mei,P Y Jiao,M Y Yao,Z N Zhuang,Z Feng.Effect of artemether on phosphorylase, lactate dehydrogenase, adenosine triphosphatase, and glucosephosphate dehydrogenase of Schistosoma japonicum harbored in mice.[J].Acta Pharmacologica Sinica,1999,20(8):750-754.
Authors:S H Xiao  J Q You  H F Guo  J Y Mei  P Y Jiao  M Y Yao  Z N Zhuang  Z Feng
Institution:Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai, China. shxiao@rocketmail.com
Abstract:AIM: To study the effect of artemether (Art) on phosphorylase (PP), lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G-6-PDH), and adenosine triphosphatase (ATPase) of S japonicum. METHODS: Mice infected with S. japonicum cercariae for 32-38 d were treated i.g. with Art 100-300 mg.kg-1 and killed 24-72 h after treatment for collection of schistosomes. The activities of PP, LDH, and G-6-PDH were measured by the formation of NADH or NADPH. The activity of ATPase was measured by the rate of release of inorganic phosphate (Pi) from ATP at 37 degrees C. RESULTS: After infected mice were treated i.g. with Art 300 mg.kg-1 for 24-48 h, the activities of total PP and PPa (active form) increased markedly in both male and female worms, while PPb (inactive form) showed no or only a slight increase. At 24-72 h after the above-mentioned mice were treated i.g. with Art 100-300 mg.kg-1, the inhibitory rates of LDH and G-6-PDH were 9%-59% (male) and 41%-75% (female) as well as 22%-42% (male) and 74%-89% (female), respectively. When Art 300 mg.kg-1 was given to infected mice for 24 h, only the activity of Mg(2+)-ATPase showed marked inhibition in both male and female worms. At 48 h, the Ca(2+)-ATPase, Mg(2+)-ATPase, and Na(+)-K(+)-ATPase were all inhibited, the inhibitory rates of 17% (male) and 19% (female), 32% (male) and 48% (female) as well as 29% (male) and 44% (female), respectively. CONCLUSION: In schistosomes, the increase in the activity of AMP-independent PPa induced by Art may enhance the decomposition of glycogen and the inhibition of LDH by Art could reduce the formation of lactate. Moreover, Art exerts a potent inhibition on the G-6-PDH activity of the female S japonicum.
Keywords:
本文献已被 维普 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号