| 藤黄酸对胃癌BGC-803细胞凋亡的作用及对survivin基因表达的影响 |
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| 引用本文： | 黄恺飞, 陆云华, 何睿, 奚涛. 藤黄酸对胃癌BGC-803细胞凋亡的作用及对survivin基因表达的影响[J]. 中国药科大学学报, 2008, 39(5): 474-478. |
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| 作者姓名： | 黄恺飞 陆云华 何睿 奚涛 |
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| 作者单位： | 1.中国药科大学生命科学与技术学院 |
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| 摘 要： | 目的:研究藤黄酸对人胃癌细胞株BGC-803凋亡的作用及其对胃癌细胞survivin基因表达的影响。观察应用RNA干扰(RNAi)技术沉默survivin基因的表达后,藤黄酸对胃癌细胞BGC-803的抑瘤效应。方法:应用MTT、DNAladder等方法研究藤黄酸对胃癌细胞系BGC-803凋亡的诱导作用,运用RT-PCR、Western-blotting分析藤黄酸和survivin-siRNA对胃癌细胞survivin表达的影响。MTT法检测藤黄酸对转染survivin-siRNA后的BGC-803细胞株抑瘤作用。结果:藤黄酸可抑制BGC-803细胞的生长,其抑制率与药物浓度及作用时间呈依赖关系。藤黄酸作用BGC-803细胞后,survivin蛋白表达降低。通过siRNA有效阻断BGC-803细胞survivin基因的表达后,藤黄酸对细胞的抑瘤效果可以进一步提高。结论:藤黄酸可以有效的抑制survivin基因表达,从而促进肿瘤细胞凋亡,同时survivin-siRNA与藤黄酸具有明显的协同抗肿瘤作用。
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| 关 键 词： | 藤黄酸 survivin基因 凋亡 BGC-803细胞 |
Gambogic acid-induced apoptosis of gastric cancer cells BGC-803 and its influence on the expression of survivin |
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| HUANG Kai-fei,LU Yun-hua,HE Rui,XI Tao. Gambogic acid-induced apoptosis of gastric cancer cells BGC-803 and its influence on the expression of survivin[J]. Journal of China Pharmaceutical University, 2008, 39(5): 474-478 |
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| Authors: | HUANG Kai-fei LU Yun-hua HE Rui XI Tao |
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| Affiliation: | HUANG Kai-fei,LU Yun-hua,HE Rui,XI Tao School of Life & Science , Technology,China Pharmaceutical University,Nanjing 210009,China |
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| Abstract: | Aim:To investigate the effects of gambogic acid induced apoptosis and the influence of gambogic acid on the expression of survivin in human gastric cancer cell line BGC-803,and to explore the inhibitory effects of gambogic acid in human gastric cancer cell line BGC-803 which was subjected to RNA interference(RNAi) to knockdown the expression of survivin gene.Methods:Methyl thiazolyl tetrazolium(MTT) and DNA ladder were used to study the effects of gambogic acid-induced apoptosis in gastric cancer cell line ... |
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| Keywords: | gambogic acid survivin gene apoptosis BGC-803 cells |
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