大鼠肝癌模型经肝动脉改良给药技术的建立

目的建立大鼠移植性肝癌模型,探讨改良的经肝动脉插管(Lindell法)给药技术在该模型上运用的可行性及价值。方法20只SD大鼠采用完整组织块肝内植入法建立模型,随机分为两组,在手术显微镜下经胃十二指肠动脉逆行细针穿刺灌注染料,其中组A采用改良的Lindell给药技术,组B采用常规Lindell法,观察大鼠各肝叶染色程度,比较两组模型肿瘤重量及体积。结果模型复制和给药成功率为100%,两组瘤重和瘤体比较差异无统计学意义(P>0.05);在灌注前暂时阻断肝固有动脉右侧分支的改良Lindell技术使大鼠尾状叶、右叶及中叶染色程度明显减少(P<0.05)。结论用完整组织块肝内植入法建立的大鼠移植性肝癌模型稳定;改良的Lindell给药技术能模拟临床肝癌介入超选择插管技术,适合于肝癌血管介入治疗的实验研究。

Establishment of improved transarterial intervention technique for hepatomagraft model on rats

Objective To establish a hepatomagraft rat model and study the feasibility and value on possible chinical application.Methods Implantation of histoloigcally intact Walker-256 tumor tissue in the left extra-lobe of liver was carried out in 20 SD rats. Ten days after implantation, rats were divided into two groups. Under binocular operative microscope, needle was retrograde inserted into the gastroduodenal artery and then infused dye into the liver lobes. Improved technique characterized by temporary block of the right branch of hepatic artery proper during injection in group A, versus the routine transarterial intervention technique in group B (Lindell's method), were carried out. Tumor volume and weight were compared before and after injection of the two groups together with the degrees of staining in liver lobes. Results The successful rates of tumor implantation and dye injection reached 100%. No sigrificant difference concerning the tumor volume and weight between the two groups were shown (P> 0.05 ). Staining degrees of caudate lobe, right lobe and middle lobe in group A were significantly mild in comparing with the rest lobes both in group A and group B (P<0.05). Conclusions The experimental model is stable and reproducible. The application of the improved transarterial administration technique presente virtual clinical superselective catheterization and might be a valuable method for experimental research in interventional therapy of hepatoma.