HPLC法测定乳酸左氧氟沙星氯化钠注射液的含量

目的：建立HPLC法测定乳酸左氧氟沙星氯化钠注射液的含量。方法：色谱柱为C18柱（150mm×4.6mm,5μm）,流动相为0.05mol/L枸橼酸溶液-1mol/L醋酸铵溶液-乙腈（77：1：14）,检测波长为293nm,流速为1.0ml/min,理论板数应不低于2500。结果：左氧氟沙星在10μg/ml～90μg/ml浓度范围内与峰面积呈良好的线性关系（r=0.9999）;平均回收率为99.28%,RSD为0.47%。结论：此方法快捷、准确,可作为乳酸左氧氟沙星氯化钠注射液质量控制的方法。

Determination of Levofloxacin Lactate Sodium Chloride Injection Content by HPLC

Objective： To establish HPLC determination of levofloxacin lactate sodium chloride injection content. Methods：The analytical column was C18 column（ 150mm × 4. 6mm,5μm） ;mobile phase： consisted of 0.05mol/L citricaeid solution - lmo]/L solution of ammonium acetate - acetonitrile（77：1：14）. The detection wavelength was 293nm, The flow rate was 1.0ml/min. Number of theoretical plates should not be less than 2500. Results：Levofloxacin in 10μg/ml - 90ug/ml the concentration range of a good linear relationship with the peak area（r = 0. 9999）. The average recovery was 99. 28%. RSD was 0. 47%. Conclusion： This method is fast, accurate and can be used as levofloxacin lactate and sodium chloride injection method of quality control.