CD44v6、v9和P53蛋白在人喉癌中的表达及与预后的关系

目的：探讨CD44v6、v9和P53蛋白联合表达在人喉癌发生、发展及预后中的作用。方法：采用免疫组织化学EnVi—sion法检测223例喉不同病变组织标本(喉癌143例，喉癌前病变32例，声带息肉39例和癌周正常喉组织9例)中CD44v6、v9和P53蛋白的表达。结果：(1)CD44v6、v9和P53在人喉癌组织中表达阳性率分别为72．7％(104／143)、56．6％(81／143)和52．4％(75／143)；喉癌前病变组织中为37．5％(12／32)、31．3(10／32)％和21．9％(7／32)；声带息肉中为17．9％(7／39)、12．8％(5／39)和10．3％(4／39)；癌周正常喉组织中均为0(0／9)；3种蛋白在喉癌组织中阳性表达率均显著高于癌前病变及声带息肉组织(P＜0．01)。(2)CD44v6、v9和P53在人喉癌组织中联合表达阳性率为41．3％(59／143)，显著高于其他喉不同病变组织中联合表达阳性率(8．8％，7／80，P＜0．01)。(3)CD44v6、v9和P53在Ⅰ—Ⅱ期喉癌组织中表达阳性率分别为56．5％(35／62)、40．3％(25／62)和38．7％(24／62)，均显著低于Ⅲ—Ⅳ期喉癌组织[分别为85．2％(69／81)、69．1％(56／81)和62．9％(51／81)，P＜o．011。(4)CD44v6、v9和P53在伴颈淋巴结转移的喉癌组中表达阳性率分别为85．7％(36／42)、78．6％(33／42)和73．8％(31／42)，均显著高于无颈淋巴结转移组[分别为67．3％(68／101)、47．5％(48／101)和43．6％(44／101)，P＜0．01]。结论：CD44v6、v9和P53联合表达与人喉癌的发生、发展及预后有一定的相关性。

Expression of CD44v6,v9 and P53 protein in laryngeal carcinoma and its relation with prognosis

Objective:To study the expression of CD44v6,v9 and P53 protein in laryngeal carcinoma and their relationship with the occurrence, development and prognosis of laryngeal carcinoma. Methods: Immunohistochemistry was used to study 143 cases of laryngeal carcinoma tissues, 32 cases of precarcinoma tissues, 39 cases of vocal cords polypus and 9 cases of normal tissues adjacent to laryngeal carcinoma. Results: (1)The positive rate of CD44v6,v9 and P53 was 72.7%(104/143),56.6%(81/143) and 52.4%(75/143) in laryngeal carcinoma, and 37.5%(12/32),31.3(10/32)% and 21.9%(7/32) in precarcinoma, 17.9%(7/39),12.8%(5/39) and 10.3%(4/39) in vocal cords polypus,all negative in normal tissues adjacent to laryngeal carcinoma.(2) The over all positive rate of CD44v6,v9 and P53 was 41.3%(59/143) in laryngeal carcinoma tissues and 8.8%(7/80) in non carcinoma tissues.(3) The positive rate of CD44v6,v9 and P53 was 85.2%(69/81), 69.1% (56/81) and 62.9%(51/81) at the third and fourth stages of laryngeal carcinoma respectively, 56.5%(35/62), 40.3% (25/62) and 38.7% (24/62) at the first and second stages. (4) The positive rate of CD44v6,v9 and P53 was 85.7%(36/42),78.6%(33/42) and 73.8%(31/42) in laryngeal carcinoma with cervical metastasis respectively, 67.3%(68/101),47.5%(48/101) and 43.6% (44/101) in those without cervical metastasis.Conclusion: The high expression of CD44v6,v9 and P53 protein may be closely related to the occurrence, development and prognosis of laryngeal carcinoma.