用PCR-SSCP方法检测TLR9基因多态性

目的探讨用多聚酶链反应-基因单链构象(PCR-SSCP)方法研究Toll样受体基因多态性。方法用PCR-SSCP方法检测82例肿瘤患者和82例健康对照人群的TLR9基因-1486、-1237、1174、2848位点的单核苷酸多态性。结果164例人群样本中,TLR9基因多态性频率分布分别是:1486、1174、2848位点在对照组和病例组的比例为0%和1.2%,1.2%和7.3%,0%和3.7%;-1237在对照组和病例组中均未发现多态性。在病例组中出现4种多态性的比例为12.2%,在对照组中为1.2%,P=0.0092,OR=11.25,95%CI为1.41~90.05。结论TLR9多态性在人群中与癌肿有一定的关系,基因突变可能导致癌肿危险度增加。

Detecting TLR9 gene polymorphism with polymerase chain reaction -single strand conformation polymorphism

Objective To explore TLR9 gene polymorphism with polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP).Methods single nucleic-acid polymorphism(SNPs,-1468,-1237,1174 and 2848)of TLR9 gene were screened by PCR-SSCP in 82 tumour cases and 82 matched controls.Results The TLR9 gene was measured in 164 blood samples from Hubei(including 82 tumour cases and 82 health controls).The frequencies of TLR9 gene SNPs: the proportions at-1486,1174 and 2848 sites between controls and cases were 0% vs 1.2%,1.2% vs 7.3%,0% vs 3.7%,respectively.At-1237 site of TLR9 gene,we did not screen polymorphism in controls and cases.The occurred possibility of one of 4 SNPs in cases was 12.2% but 1.2% in controls(P=0.0092,OR=11.25,95% CI 1.41～90.05).The incidences of four SNPs in 164 samples were 0.6%,0%,4.3% and 1.8%,respectively.Conclusion In Hubei,China population,there may be some relations between TLR9 gene polymorphism and tumor.The mutations of TLR9 gene can increase the risk of cancer.