人腺病毒3型感染对H9c2细胞microRNA表达谱的影响

目的研究腺病毒感染对大鼠心肌来源的H9c2细胞microRNA表达谱的影响。方法用人腺病毒3型（Ad3）攻击成层和未成层的H9c2细胞，6h后收获总RNA，基因芯片检测microRNA表达谱。结果比较未成层与成层的H9c2细胞microRNA的表达谱，H9c2细胞成层后有54种microRNA表达显著下调，7种microRNA表达显著上调。与未感染病毒的细胞比较，Ad3感染的未成层H9c2细胞有41种microRNA表达显著下调，12种microRNA表达显著上调。与未感染病毒的细胞比较，Ad3感染成层H9c2细胞后有8种microRNA的表达显著下调，62种microRNA的表达显著上调。结论细胞不同的生长状态及Ad3感染显著改变了H9c2细胞的microRNA表达谱。

microRNA expression profile of H9c2 cells infected with human adenovirus type 3

Objective To study the microRNA expression profile of rat myoeardium-derived H9c2 cells in-fected with human adenovirus type 3(Ad3).Methods Confluent and unconfluent H9c2 cells were attacked with Ad3.Six hour later total RNAs were harvested and microRNA expression profiling was analyzed by mi-cmRNA array.Results The expression of 54 microRNAs Was down-regulated and 7 micwRNAs Was up-reg-ulated in COnnuent H9c2 cells compared with that in unconiluent H9c2 cells.The expression of 41 miemR-NAs wag down-regulated and 12 Was up-regulated in unconfluent H9e2 cells infected with Ad3 compared with that in unconiluent H9c2 cells without Ad3 infection.There were 8 microRNAs down-regulated and 62 up-regulated in confluent H9c2 infected with Ad3 compared with that in comquent H9c2 ceils without Ad3 infec-tion.Condusion The microRNA expression profile,correlating with different cell growth states,was siguif-icanfly altered by human adenovirus infection.