Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus |
| |
| Authors: | Daria Prokhorova Natalya Zhukova (Eschenko) Anna Lemza Mariia Sergeeva Rinat Amirkhanov Grigory Stepanov |
| |
| Affiliation: | 1.Laboratory of Genome Editing, Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia; (D.P.); (N.Z.); (A.L.); (M.S.); (R.A.);2.Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia;3.Laboratory of Vector Vaccines, Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation, 197376 Saint Petersburg, Russia |
| |
| Abstract: | Influenza A virus (IAV) causes a respiratory infection that affects millions of people of different age groups and can lead to acute respiratory distress syndrome. Currently, host genes, receptors, and other cellular components critical for IAV replication are actively studied. One of the most convenient and accessible genome-editing tools to facilitate these studies is the CRISPR/Cas9 system. This tool allows for regulating the expression of both viral and host cell genes to enhance or impair viral entry and replication. This review considers the effect of the genome editing system on specific target genes in cells (human and chicken) in terms of subsequent changes in the influenza virus life cycle and the efficiency of virus particle production. |
| |
| Keywords: | CRISPR/Cas9 influenza virus cellular immune response cell receptors RNA processing factors CRISPR/Cas9 genome screening |
|
|
