Insulin-like growth factor-I and insulin-like growth factor-II induce c-fos in mouse osteoblastic cells |
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Authors: | Harold L Merriman Donn La Tour Thomas A Linkhart Subburaman Mohan David J Baylink Donna D Strong PhD |
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Institution: | (1) Department of Biochemistry, Loma Linda University and Jerry L. Pettis Veterans Administration Hospital, 11201 Benton Street, Loma Linda, California, USA;(2) Department of Medicine, Loma Linda University and Jerry L. Pettis Veterans Administration Hospital, 11201 Benton Street, 92357 Loma Linda, California, USA;(3) Department of Pediatrics, Loma Linda University and Jerry L. Pettis Veterans Administration Hospital, 11201 Benton Street, 92357 Loma Linda, California, USA;(4) Department of Physiology, Loma Linda University and Jerry L. Pettis Veterans Administration Hospital, 11201 Benton Street, 92357 Loma Linda, California, USA |
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Abstract: | Summary We investigated the expression of c-fos in mouse osteoblast-like cultures treated with insulin-like growth factor (IGF)-I
and IGF-II. The IGFs are present in bone, are produced by osteoblast-like cells in culture, and stimulate osteoblast cell
proliferation. Quiescent, subconfluent cultures of the clonal osteoblast-like mouse calvarial cell line, MC3T3-E1, were treated
with 10 ng/ml of IGF-I or IGF-II. RNA was extracted at 0, 15, 30, 60, 120 and 240 minutes, and c-fos messenger RNA (mRNA)
was analyzed on Northern blots. Both IGFs transiently increased c-fos mRNA levels 25–28 fold at 15–30 min. To determine if
c-fos induction was unique to the MC3T3-E1 cell line, effects of IGF-1 and IGF-II (3 ng/ml) were also tested in quiescent,
serum-free primary mouse calvarial cells. Levels of c-fos mRNA were increased at 15 and 30 minutes (40-fold with IGF-I and
5-fold with IGF-II). These results indicate that IGF-I and IGF-II caused a rapid and transient induction of c-fos mRNA in
murine osteoblasts. |
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Keywords: | IGF-I IGF-II c-fos Primary mouse calvarial cells MC3T3-E1 cells Osteoblasts |
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