MMP9及E-cadherin在结直肠癌中的表达及意义

目的:研究基质金属蛋白酶(matrix metalloproteinase-9,MMP 9)、上皮钙黏附素(E-cadherin,E-CD)蛋白在结直肠癌中的表达,探讨其与结直肠癌浸润、转移的关系,以及两者的表达特征和内在联系。方法:应用免疫组化技术SP法检测MMP9和E-cadherin蛋白在52例结直肠癌中的表达情况(其中3例为腺瘤癌变),同时以10例非肿瘤性肠组织作对照。结果:MMP9与淋巴结转移及浸润密切相关。淋巴结转移组(100%)明显高于无淋巴结转移组(89%),生存期<5年的患者组的MMP9过表达显著高于生存期≥5年的患者组(P<0.05)。E-CD表达与有无淋巴结转移相关(P<0.01),与结直肠癌的细胞分化程度、Dukes分期无明显相关性(P>0.05)。结论:MMP9的高表达及E-cadherin失表达或表达减低与结直肠癌转移浸润有密切关系。MMP9的阳性表达与E-cadherin异常表达密切相关。

Expressions of MMP-9 and E-cadherin in colorectal carcinoma and their clinical significance

Objective:To investigate the expressions of matrix metalloproteinase-9(MMP-9) and E-cadherin protein in human colorectal carcinoma and their correlation with tumor infiltration and metastasis as well as evaluate the relationship between the expressions of the two proteins.Methods:The expressions of MMP-9 and E-cadherin protein were detected by immunohistochemical staining(streptavidine-peroxidase method) in 52 cases of colorectal carcinoma(including 3 cases of colorectal adenomas with malignant change).Ten cases of normal colorectal tissues were as control.Results:(1) The over-expression of MMP-9 was closely related with lymph node metastasis and invasion of colorectal carcinoma.The positive rate of MMP-9 expression in the patients with lymph node metastasis(100%) was significantly higher than those without lymph node metastasis(89%).The positive expression of MMP-9 was significantly higher in patients who survived less than 5 years than those who survived more than 5 years(P<0.05).(2) The expression of E-cadherin protein was closely associated with the lymph node metastasis(P<0.05) but it had no significant correlation with tumor differentiation and Dukes' stages(P>0.05).Conclusion:Over-expression of MMP-9 and reduction or loss of E-cadherin expression has significant correlation with metastasis and infiltration of colorectal carcinoma.The positive expression of MMP-9 closely correlated with the abnormal expression of E-cadherin.