脑内皮细胞趋化因子受体5 参与β-淀粉样蛋白引起的T细胞跨内皮迁移

 目的利用血-脑屏障的体外模型，分析脑内皮细胞上的趋化因子受体5（CCR5）在β淀粉样蛋白（Aβ）引起的T 细胞跨内皮迁移过程中的作用。方法培养人脑微血管内皮细胞（HBMEC），建立体外血-脑屏障模型，分析T 淋巴细胞跨内皮迁移的能力。结果Aβ能够促进T淋巴细胞穿过体外血-脑屏障模型，HBMEC 中CCR5 过表达能够促进T淋巴细胞穿过体外血脑屏障模型，HBMEC 中CCR5 缺失突变能够抑制T淋巴细胞穿过体外血-脑屏障模型。结论HBMEC 中CCR5 参与Aβ引起的
T淋巴细胞的跨内皮迁移。

Chemokine Receptor 5 of Human Brain Microvascular Endothelial Cells Takes Part in Amyloid beta Induced T Lymphocytes Transendothelial Migration

AbstractObjective To investigate the function of chemokine receptor 5 （CCR5）in human brain microvascular endothelial cells（HBMEC）during T lymphocytes transendothelial migration induced by amyloid beta（Aβ）. Methods The in vitro model of blood-brain barrier was established with cultured HBMEC monolayer. The ability of T lymphocytes transendothelial migration induced by Aβwas analyzed by Transwell and Millicell-ERS endothelia volt-ohmmeter. Results Aβcould promote T lymphocytes to migrate through the HBMEC monolayer. Over-expression of CCR5 in HBMEC promoted T lymphocytes to migrate through HBMEC monolayer. Expression of CCR5 mutant in HBMEC inhibited the transendothelial migration of T lymphocytes. Conclusion CCR5 of HBMEC is involved in T lymphocytes transendothe lial migration induced by Aβ.