| 应用细胞芯片捕获人正常胃细胞系、胃癌细胞系细胞并检测其细胞表面CD表型的差异 |
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| 引用本文： | 马静红,何群,徐晓雪,王天骄,潘忠诚,钟连声,张玉魁,赵雨杰.应用细胞芯片捕获人正常胃细胞系、胃癌细胞系细胞并检测其细胞表面CD表型的差异[J].中国医科大学学报,2010,39(7). |
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| 作者姓名： | 马静红 何群 徐晓雪 王天骄 潘忠诚 钟连声 张玉魁 赵雨杰 |
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| 作者单位： | 1. 中国医科大学,卫生部细胞生物学重点实验室,生物芯片中心,沈阳,110001
2. 中国医科大学,神经病学研究所,沈阳,110001 |
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| 基金项目： | 国家自然科学基金资助项目(20672144) |
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| 摘 要： | 目的 构建一种细胞芯片,根据芯片表面固定的CD抗体与细胞膜表面CD抗原免疫结合原理捕获细胞,检测人正常胃黏膜细胞系GES-1、胃腺癌细胞系7901细胞表面CD抗原表达的差异.方法 将64种CD抗体蛋白固定在化学修饰醛基玻片上制成抗体阵列,将GES-1、7901细胞用胶原酶消化制成单个细胞悬液,用吖啶橙荧光标记后与芯片进行孵育,洗去芯片上游离的细胞,激光扫描仪检测芯片上CD抗体各点捕获到细胞的荧光信号,以此判定GES-1、7901细胞膜表面CD表型情况.结果 发现CD9、CD13、CD29、CD44、CD49、CD55、CD71、CD95抗体点均捕获到GES-1、7901细胞;CD15、CD24抗体点仅捕获到GES-1细胞;CD77、CD133、CD103、CD64抗体点仅捕获到7901细胞.结论 CD15和CD24可能是人正常胃黏膜细胞系GES-1细胞的表面标志物,CD77、CD133、CD103、CD64可能是人胃癌细胞系7901细胞的标志物.
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| 关 键 词： | 细胞芯片 CD抗体 GES-1细胞 7901细胞 |
Microarray Capture of Human Normal Gastric Cell Line and Gastric Cancer Cell Line and Diversities of Cell-surface CD Antigens |
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| MA Jing-hong
,
HE Qun
,
XU Xiao-xue
,
WANG Tian-jiao
,
PAN Zhong-cheng
,
ZHONG Lian-sheng
,
ZHANG Yu-kui
,
ZHAO Yu-jie.Microarray Capture of Human Normal Gastric Cell Line and Gastric Cancer Cell Line and Diversities of Cell-surface CD Antigens[J].Journal of China Medical University,2010,39(7). |
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| Authors: | MA Jing-hong HE Qun XU Xiao-xue WANG Tian-jiao PAN Zhong-cheng ZHONG Lian-sheng ZHANG Yu-kui ZHAO Yu-jie |
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| Institution: | MA Jing-hong1,HE Qun1,XU Xiao-xue2,WANG Tian-jiao1,PAN Zhong-cheng1,ZHONG Lian-sheng1,ZHANG Yukui1,ZHAO Yu-jie1(1.Biochip Center,Key Laboratory of Ministry of Health,2.Institute of Neurology,China Medical University,Shenyang 110001,China) |
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| Abstract: | Objective To construct a cell microarray based on specificity of antigen-antibody reaction and by fixing monoclonal antibodies against CD antigens(CD antibodies)on the microarray substrate,and to screen cell-surface CD antigens in normal gastric cell line GES-1 and gastric cancer cell line 7901.Methods A total of 64 kinds of CD antibodies were spotted on the aldehyde slide to construct the cell microarray.GES-1 and 7901 cells were isolated,labeled with fluorescence,and captured by the cell microarray.The ce... |
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| Keywords: | cell microarray CD antibody GES-1 cell 7901 cell |
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