| 左旋多巴固体脂质纳米粒的制备及包封率测定 |
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| 引用本文： | 詹水明,侯冬枝,平其能,徐岩.左旋多巴固体脂质纳米粒的制备及包封率测定[J].中国医院药学杂志,2010(14). |
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| 作者姓名： | 詹水明 侯冬枝 平其能 徐岩 |
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| 作者单位： | 广东药学院药科学院;中国药科大学药学院;华中科技大学附属协和医院; |
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| 基金项目： | 国家自然科学基金(编号:30700881); 广东药学院人才引进科研启动基金项目(编号:2007YKX20) |
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| 摘 要： | 目的:用微乳法制备左旋多巴固体脂质纳米粒(LDP-SLN),并建立包封率的测定方法。方法:通过绘制三元相图,采用微乳法制备LDP-SLN,用TEM和激光粒度仪进行了颗粒形貌和粒径分布的研究,用葡聚糖凝胶层析法分离测定包封率。对其粒径、形态、包封率等理化性质进行研究,并考察其稳定性。结果:实验制得LDP-SLN为稳定的略泛蓝色乳光的纳米混悬液,在透射电镜下显示为较为均匀的球体,激光粒度测定平均粒径为108nm,多分散系数1.153;4℃放置2个月,粒径、包封率无显著变化。包封率测定的线性范围为2～100mg·mL-1,线性良好(r=0.9999),精密度符合要求,LDP-SLN上柱洗脱后分离度和回收率均符合要求。结论:该研究中制备了物理性质较为稳定的LDP-SLN,建立了合适的包封率测定方法,并考查初步稳定性较好。
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| 关 键 词： | 左旋多巴 固体脂质纳米粒 包封率 |
Preparation and entrapment efficiency determination of solid lipid nanoparticles loaded levodopa |
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| ZHAN Shui-ming,HOU Dong-zhi,PING Qi-neng,XU Yan.Preparation and entrapment efficiency determination of solid lipid nanoparticles loaded levodopa[J].Chinese Journal of Hospital Pharmacy,2010(14). |
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| Authors: | ZHAN Shui-ming HOU Dong-zhi PING Qi-neng XU Yan |
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| Institution: | ZHAN Shui-ming1,HOU Dong-zhi1,PING Qi-neng2,XU Yan3(1.Dept.of Pharmaceutical Sciences,GuangDong College of Pharmacy,Guangdong Guangzhou 510006,China,2.College of Pharmacy,China Pharmaceutical University,Jiangsu Nanjing 210009,3.Xiehe Hospital,Huazhong University of Science & Technology,Hubei Wuhan 430070,China) |
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| Abstract: | OBJECTIVE Levodopa solid lipid nano-particles(LDP-SLN)was prepared,and an efficient method for determination of entrapment efficiency(EE%)was established.METHODS LDP-SLN was prepared through the micro-emulsion technology prepared by drawing ternary phase diagram,The morphology was examined by transmission electron microscope,the particle size was determined by laser granularity equipment,and the entrapment efficiency(EE%)was determined with the Sephadex gel chromatogram.The mean particle size,drug entrapmen... |
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| Keywords: | levodopa solid lipid nanoparticles entrapment efficiency |
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