广西HCV高危人群庚型肝炎病毒的新基因型核苷酸序列分析

目的 探讨广西HCv高危人群庚型肝炎病毒(HGv)的感染情况及其新基因型的核苷酸序列。方法 静脉药瘾者(IVDAs)85份、肝病患者(PLDs)80份和献血员(BDs)50份血清标本．用PCR法检测庚型肝炎病毒RNA，EIA法检测HBsAg、抗-HCV和抗-HIV；随机选出62份庚型肝炎病毒RNA阳性标本进行核苷酸序列分析，构建种系发生树作基因分型。结果 215份血清中HGv阳性者85份(39.53％)，HBsAg、抗-HGV和抗-HIV的阳性率分别为39.07％、42．79％和0；11份HGV RNA的测序结果证实其有3种基因型，其中5株为新基因型(亚洲型)，51份补测序，其中GBV—C型占3．23％，HGV型占30.65％，亚洲型占64．51％。结论 HGV的3种基因型中存在不同的基因亚型；广西IVDAs、PLDs和BDs中感染庚型肝炎病毒以亚洲型和HGV型为主。

Nucleotide sequence analysis of new genotype of hepatitis G virus in population at high risk for HCV infection in Guangxi

Objective To examine the prevalence and the sequence of the genes of new genotypes of hepatitis G virus (HGV) in Guangxi, China. Methods Serum samples were collected from 85 intravenous drug abusers (IVDAs), 80 patients with liver diseases (PLDs) and 50 blood donors (BDs). All sera ( n =215) were tested by using EIA for HBsAg,anti HCV and anti HIV, and by using nested PCR for HGV RNA. In 62 subjects positive for HGV, HGV RNA was sequenced, and a phylogenetic tree was constructed for analyzing genotypes of HGV.Results HGV RNA was detected in 85 of 215 serum samples (39.53%). The positivity rates for HBsAg, anti HCV and anti HIV were 39.07%,42.79% and 0, respectively. First, 11 nucleotide sequences were determined and the isolates were grouped into three clusters with HGV. 5 of 11 HGV isolates clustered in a distinct phylogenetic branch (genotype Asia)which was different from the described GBV C and HGV sequences, suggesting the presence of a new genotype of HGV in this locality. Second, 51 nucleotide sequences were determined and analyzed for their genotypes of HGV, and showed genotype GBV C (3.23%),genotype HGV 30 65% and new genotype (genotype Asia)64.51%,respectively. Conclusion There were subgenotypes in 3 genotypes of HGV;The predominant genotypes of HGV were genotype Asia and genotype HGV among IVDAs, PLDs, and BDs patients in Guangxi, China.