SiO2对大鼠肺泡巨噬细胞原癌基因c-sis表达影响的体外研究

目的　探讨SiO2 对大鼠肺泡巨噬细胞原癌基因c sis表达的影响以期进一步了解原癌基因c sis在矽肺发病中的作用。方法　采用支气管肺泡灌洗方法获得肺泡巨噬细胞 (alveolarmacrophage ,AM)。体外予SiO2 对AM进行不同时间的处理 ,利用RT PCR方法检测c sismRNA表达 ,结果用计算机自动分析系统进行定量分析。结果　AM中c sis在SiO2 处理后存在高表达 ,处理时间不同其表达量也不同 ,3 0min即有表达 ,1h表达达高峰 ,以后随处理时间的延长表达量逐渐下降 ,各时间点结果与不加SiO2 处理的同期对照组相比差异均有显著性 (P <0 0 1)。结论　SiO2 可诱导AM原癌基因c sis出现高表达 ,提示原癌基因c sis可能参与矽肺纤维化早期的形成。

Study on the effect of SiO2 on the expression of pro-oncogene c-sis in rat alveolar macrophages in vitro

Objective To study the effect of SiO 2 on the expression of pro-oncogene c-sis in rat alveolar macrophages in order to know the function of c-sis in forming of silicosis.Method Alveolar macrophages were collected by bronchoalveolar lavage and silica particles were alveolav macrophages culture media.RT-PCR was used to detect the expression of c-sis,the results were analyzed quantitatively by Bio-Rad 100 gel-image density analytical system.Result Alveolar macrophages treated by SiO 2 could induce c-sis mRNA excessive express,at 1 hour after SiO 2 exposure their express quantity reached peak. Compared with control,their expressed quantities at different culture times were all more than that of controls.Conclusion SiO 2 could induce alveolar macrophages c-sis excessive express and which might play an important role in forming of the silicosis.