超声介导下携尿激酶靶向微泡在兔动脉内溶栓后的电镜观察

目的 探讨携尿激酶靶向微泡溶解兔动脉内血栓后下的形态学改变。方法 在24只新西兰大白兔单侧股动脉制作富含血小板的混合性血栓，分成4组，每组6只:①单纯超声照射组(US);②单纯尿激酶静脉注射组(UK);③超声照射+微泡造影剂+尿激酶静脉注射组(US+ M+ UK);④超声照射+RGDS微泡造影剂+尿激酶组(US+ R+ UK)。将RGDS、微泡造影剂(SonoVue)和尿激酶通过直接联合法配制成携尿激酶的靶向微泡，超声照射30 min，多普勒血流仪监测血流量变化评价溶栓效果，然后对其行HE染色、电镜检查。结果 US+ R+ UK组血流量完全恢复，实现完全再通（P＜0.001），HE染色显示血栓完全溶解，扫描电镜检查示血栓的纤维网状结构破坏，透射电镜显示血栓的大量降解碎片。结论血栓纤维蛋白网状结构的破坏、纤维蛋白的溶解是携尿激酶靶向微泡在兔动脉内溶解血栓的主要电镜表现。

Observation of urokinase targeted microbubbles dissolved the thromb in vivo by electron microscope

Objective To study the change of thrombosis in rabbits arteries by electron microscope after thromb was lyses by urokinase targeted microbubbles.Methods A total of 24 rabbits with plateletrich thrombi in the femoral artery were randomized into 4 treatment groups (n = 6) :(1) ultrasound alone (US) ; (2) urokinase alone (UK); (3) ultrasound, non-targeted microbubble and urokinase (US + M + UK) ;(4) ultrasound, platelet-targeted microbubble and urokinase (US + R + UK).Urokinase targeted microbubbles were in conjunction with the surface of commercial microbubbles (SonoVue) by direct conjugation method.US was simultaneously applied transcutaneously over the thrombus up to 30 min.The thrombolytic effect was based on ultrasound, blood flow and histological observations at 120 min post treatment.Results US + R + UK group was completed recanalization (P ＜ 0.001).Scanning electron microscope examination showed thrombosis of the fiber network structure damage.Conclusions The main electron microscope change of RGDS urokinase targeted microbubbles dissolve thromb is thrombus fibrin network structure damage and fibrin dissolution.