维生素E琥珀酸酯诱导人食管癌Eca-109细胞凋亡

 目的 研究维生素E琥珀酸酯(VES）对人食管癌Eca-109细胞株增殖、凋亡及相关蛋白表达的影响。方法 实验组为不同浓度VES，对照组为同等体积RPMI-1640培养基，分别作用24、48和72h后，MTT测细胞增殖；电镜观察细胞凋亡的形态学；流式细胞术（FCM）测凋亡率及Survivin、Caspase-3蛋白表达。结果 各实验组抑制率随VES处理浓度的增加、作用时间的延长而逐渐升高 (P <0.01)。实验组细胞呈现不同程度凋亡改变，且随VES浓度增加，凋亡率逐渐升高 (P <0.05)。各实验组Survivin蛋白表达减少(P <0.05)，Caspase-3蛋白表达增加(P <0.05)。Survivin与Caspase-3表达呈负相关(r=-0.95, P <0.05)。结论 VES可抑制Eca-109细胞增殖、诱导凋亡，可能与下调Survivin蛋白和上调Caspase-3蛋白表达有关。

Vitamin E succinate induces the apoptosis of carcinoma of esophagus cell line Eca-109

Objective To study the effects of Vitamin E Succinate(VES) on proliferation, apoptosis and expressions of associated protein of human esophageal carcinoma cell line Eca-109.Methods Experimental groups were VES of different contrations and control group was culture medium in same volume. After Eca-109 cells were exposed for 24h,48h and 72h, MTT was used to measure the proliferation of cells.Morphological changes of Eca-109 cells were observed by transmission electron microscopy. Apoptotic rate and the expression of Survivin, Caspase-3 protein of Eca-109 among different concentration groups was examined by flow cytometry. Results The inhibitory rate increased gradually,with the increasing concentration of Vitamin E Succinate and prolonging of treatment time(P<0.01).Apoptosis morphous change appeared in different degree in cells. With the increasing concentration of VES,the apoptotic rate of every treatment group was significantly higher than that of control group(P<0.05). The protein expression of Survivin in Eca-109 reduced graduately(p<0.05),but Caspase-3 increased graduately(p<0.05). These two proteins had inverse correlation(r=-0.95, P <0.05). Conclusion: Vitamin E Succinate can inhibite the proliferation and induce apoptosis of Eca-109 cell line.The effect may be ralated to up-regulation the expression of Caspase-3 protein and down-regulation of the expression of Survivin.