| FHIT基因与喉癌相关性的研究 |
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| 引用本文： | 富伟能,吴东宁,张学. FHIT基因与喉癌相关性的研究[J]. 中华肿瘤杂志, 1999, 21(6): 436-438 |
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| 作者姓名： | 富伟能 吴东宁 张学 |
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| 作者单位： | 中国医科大学医学遗传学教研室(富伟能!110001沈阳,吴东宁!110001沈阳,张学!110001沈阳,金春元!110001沈阳,崔勇志!110001沈阳,孙开来!110001沈阳),解放军第四六三医院(徐振明) |
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| 摘 要： | 目的 研究ＦＨＩＴ基因在喉癌发生中的作用。方法 应用ＲＴ－ＰＣＲ技术筛查了喉细胞系Ｈｅｐ－２,宫颈癌细胞系ＨｅＬａ和２０例原发性喉癌的ＦＨＩＴ基因转录本,对其中的２例异常转录本进行了测序分析。同时,对另外６０例原发性喉癌ＦＨＩＴ基因进行了微卫星多态分析。结果 在２０例喉癌中,１４例,显示ＦＨＩＴ基因异常转录本,Ｈｅｐ－２和ＨｅＬａ细胞系ＦＨＴ基因转录本均存在缺失。
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| 关 键 词： | 喉肿瘤 FHIT基因 微卫星多态性 |
FHIT gene is abnormal in laryngeal carcinoma |
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| FU Weineng,WU Dongning,ZHANG Xue,et al.. FHIT gene is abnormal in laryngeal carcinoma[J]. Chinese Journal of Oncology, 1999, 21(6): 436-438 |
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| Authors: | FU Weineng WU Dongning ZHANG Xue et al. |
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| Affiliation: | Department of Medical Genetics, China Medical University, Shenyang 110001. |
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| Abstract: | Objective To determine the role of FHIT gene in laryngeal carcinogenesis. Methods Laryngeal cancer cell line Hep 2, HeLa cell line and 80 primary tumors from patients with laryngeal cancer were studied by means of RT PCR, sequencing and microsatellite analysis. Results Abnormal transcripts of FHIT gene were found in the two cell lines and 70%(14/20) of primary laryngeal carcinomas. Aberrant laryngeal tumor transcripts often lack more than two exons of FHIT gene. Microsatellite analysis showed that both loss of heterozygosity and microsatellite instability of FHIT gene existed in laryngeal carcinoma and the former was more common. Conclusion FHIT gene participates in the laryngeal carcinogenesis and may be one of the candidate tumor suppressor genes. |
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| Keywords: | Laryngeal neoplasms FHIT gene Microsatellite polymorphism |
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