消化道类癌的免疫组化与DNA定量分析

目的 探讨消化道类癌临床病理与抗原表达、ＤＮＡ含量的关系。方法 ５２例类癌按照Ｇｏｕｌｄ方法被分为３型：典型类癌２０例，非典型类癌２０例，低分化类癌１２例。３型同时进行１０种抗原标记免疫组化染色和流式细胞仪细胞核ＤＮＡ定量分析。结果 血管活性肽（ＶＩＰ）、降钙素（ＣＴ）在典型类癌组中的分布低于其他两型，差异有显著性（Ｐ〈０．０５）；癌胚抗原（ＣＥＡ）在典型类癌中的分布低于其他两型，差异有非常显著性

Immunohistochemistry and DNA content of gastro intestinal carcinoid

OBJECTIVE: To study the relationship between clinical pathology and antigen expression and DNA content of gastrointestinal carcinoid tumors. METHODS: Fifty-two cases of paraffin-embedded gastrointestinal carcinoid specimens were collected. According to Gould's criteria, there were 20 cases of typical carcinoid, 20 cases of atypical carcinoid and 12 cases of undifferentiated carcinoid. Ten different kinds of antigen were examined by immunohistochemical staining and DNA contents by flow cytometry. RESULTS: Calcitonin (CT), carcinoembryonic antigen (CEA) and vasoactive intestinal peptide (VIP) expression was significantly reduced in typical carcionoid compared to that in other two types of carcionoid. Their expression was more marked in progression than in early stage of the disease. VIP expression was significantly higher in patients with than without lymph node metastases. Cellular DNA analysis showed significant difference between the typical and the other two types of carcinoid. Tumors with expression of VIP and CEA were mostly aneuploid. CONCLUSION: To study expression of relevant antigens in gastrointestinal carcinoid in relation to histological type and cellular DNA content helps determine its biologic behavior and prognosis.