食管鳞状细胞癌中p16基因变异的临床意义及地域性差异

目的 进一步了解Ｐ１６基因的变异与食管癌发生发展和生物学行为的关系及地域性差异。方法 采用免疫组化和聚合酶链反应技术，对来自林县高发区和浙江省的食管癌存档组织标本中Ｐ１６蛋白的表达及其因变异进行了检测，并对食管癌组织中ｐ１６基因变异表达的临床意义及地域性差异进行了回顾性比较分析。结果 ９２例食管癌组织中，４４例存在ｐ１６蛋白表达，２２例检测到ｐ１６基因的丢失，只有５例出现ＰＣＲ－ＳＳＣＰ的异常电泳

Multiple tumor suppressor 1/p16 gene alterations in human esophageal squamous cell carcinoma: clinical significance and regional difference

Objective To further understand multiple tumor suppressor 1/p16 (mts1/p16) gene alterations in squamous cell esophageal carcinoma (EC) in relation to its biologic behavior and regional difference. Methods Samples of human EC from 92 patients residing in Zhejiang province and in Linxian, Henan province were analyzed for the expression, mutation and deletion of the multiple tumor suppressor gene (mts1/p16) by immunohistochemical staining, polymerase chain reaction and single strand conformation polymorphism. Results mts1/p16 expression at protein level was demonstrated in 44 cases (47.8%). Gene deletion was detected in 22 cases (23.9%) and point mutation in 5 cases (5.4%). Expression of mst1/p16 showed significant correlation with pathologic staging, lymph node status and survival. However, deletion of mst1/p16 did not show correlation with the clinico pathologic parameters. EC patients in stage T 2 from Linxian had significantly lower rate of mst1/p16 protein expression compared to those from Zhejiang province at the same time period. Moreover, there was close association between gene alterations (mutation deletion) and frequency of lymph node metastasis in Zhejiang EC patients. No such association was observed in EC patients from Linxian. Conclusion msts1/p16 gene alteration is a common genetic event in the carcinogenesis and progression of human esophageal cancer. EC patients from two different regions present differential alterations in mst1/p16 gene.