乳腺癌表达细胞周期蛋白依赖性激酶抑制物p27kip1的预后意义

研究ｐ２７＾ｋｉｐｌ在乳腺癌组织中的表达水平，及其与生物学行为的关系和对预后的作用。方法以免疫组化法检测１８１例乳腺癌组织ｐ２７＾ｋｉｐｌ蛋白表达水平，其中９１例进行了凋亡指数、凋亡相关蛋白ｂｃｌ－２、ｂａｘ的测定，另有１０６例检测了ｐ２１＾ＷＡＦ１／ＣＩＰＩ、ｐ５３、ｍｄｍ２蛋白表达水平。结果本组１８１例中，ｐ２７＾ｋｉｐｌ蛋白阳性１２５例，占６９．１％；低表达者１２１例，占６６．９％；高表达者

Prognostic significance of cyclin dependent kinase inhibitor p27 kip1 expression in human breast cancer

Objective To study the expression of p27 kip1 in breast cancer in relation to biological behavior and prognosis. Methods p27 kip1 expression at protein level was determined by immunohistochemistry technique in 181 patients with breast cancer. In addition, p27 WAF1/CIP1 , p53 and mdm 2 were examined in 106 cases of this series. Data about apoptotic index, bcl 2 and bax protein expression were available in the other 91 patients.Results Of the 181 patients, p27 kip1 expression was low in 121 (66.9%) and high in 60 (33.1%), respectively. p27 kip1 expression level was correlated with histological grade, tumor size, axillary lymph node status, post operative recurrence, invasion and metastasis. It was also correlated with bcl 2, bax, p53, p21 WAF1/CIP1 , and mdm 2 expression levels. Univariate analysis demonstrated better disease free survival in patients with higher p27 kip1 expression than in those with lower expression. However, by Cox multivariate analysis, p27 kip1 expression level was not found to be of independent prognostic significance. Conclusion As an inhibitor of cyclin dependent kinase, p27 kip1 favors prognosis of human breast cancer not only by arresting cell cycle in G 1 phase but also inducing cancer cell apoptosis.