CD44v和透明质酸介导的细胞游走受体在多阶段胃癌发生中的表达特点

目的 探讨透明质酸（ＨＡ）受体ＣＤ４４和透明质酸介导的细胞游走受体（ＲＨＡＭＭ）在多阶段胃癌发生过程中的表达情况及其肿瘤学意义。方法 以非癌粘膜，各类癌前病变和胃癌标本为研究对象，采用常规和组织选择性方法提取样本ＲＮＡ和蛋白质，进而分析其ＣＤ４４和ＲＨＡＭＭ基因表达的特点，结果 含变异型外显子/５的ＣＤ４４广泛存在于各类组织中，ｖ７常见于非癌细胞，但随癌前病变的进展而趋降低，ｖ８～ｖ１０仅见于部分肠

Characteristics of expression of CD44v and receptor for HA mediated motility (RHAMM) in multi step gastrocarcinogenesis

OBJECTIVE: To explore the potential significance of expression of CD44 and RHAMM at different stages of gastric carcinogenesis. METHODS: The expression pattern of CD44 and RHAMM in non-cancerous mucosa, preneoplastic lesions and carcinomas of the stomach was examined by RT-PCR, Southern blot hybridization, immunohistochemical staining and Western blot hybridization. RESULTS: Expression of CD44 v5 could be observed not only in cancers, but also in pre-neoplastic and non-cancerous specimens. CD44 v7 was commonly seen in non-cancerous lesions but its expression tended to decrease with the progression of pre-malignant phenotype. CD44 v8-v10 could only be observed in some intestinal metaplasia, dysplastic proliferation and undifferentiated carcinoma. Nevertheless, CD44 v6 was found exclusively in cancer tissues, with a positive rate of 14%. The frequency of RHAMM detection was about 10% in non-cancerous mucosa but tended to increase with tumor development, reaching an average of 74% in gastric cancers. In 80% of the invading cancer cells, RHAMM and CD44v coexpressed, or complementarily expressed. CONCLUSION: Expression of RHAMM is more associated with progression of gastric malignancy. Irrespective of its low frequency, CD44 v6 is still a reliable marker of malignant transformation of gastric mucosa. Co-expression or complementary expression of CD44v and RHAMM might facilitate invasion and metastasis of gastric cancer.