复方甘正浸膏对非酒精性脂肪性肝炎CYP2E1表达的干预

目的 观察复方甘正浸膏对高脂饮食建立的非酒精性脂肪性肝炎（NASH）大鼠模型肝脏细胞色素P4502E1（CYP2E1）表达的影响。方法 用全自动生化分析仪及免疫组织化学链霉菌抗生物素过氧化物酶（S—P）法检测大鼠肝匀浆超氧化物歧化酶（SOD）活性、丙二醛（MDA）水平及肝组织CYP2E1的表达。结果 模型组SOD活性较正常组明显降低，复方甘正浸膏治疗组（治疗组）SOD活性较模型组显著增高，但仍低于正常组；模型组MDA水平较正常组显著增高，治疗组MDA水平较模型组显著降低，但仍高于正常组；CYP2E1在肝组织中央静脉周围腺泡区肝细胞胞浆表达，模型组表达阳性率70％，正常组几乎无表达，治疗组阳性率（20％）较模型组明显减少，与正常组相近。结论 复方甘正浸膏具有清除脂质过氧化和氧自由基的作用。

Effects of Compound Ganzheng on Expression CYP2E1 in Nonalcoholic Steatohepatitics

Objective： To investigate the effects of compound Ganzheng on expression of CYP2E1 in rat with nonalcoholic steatohepatitics（NASH） induced by high fat diet. Methods： The activity of SOD, the value of MDA and the expression of CYP2E1 in liver homogenates was detected by immunohistoehemistry with automatic biochemical analystor. Results： The actvity of SOD of model group was significantly lower than that of normal group, the activity of SOD of treatment group treated with compound Ganzheng was significantly higher than that of model group, and lower than that of normal group. The value of MDA of model group was significantly higher than that of normal gorup, the value of MDA of treatment group treated with compound Ganzheng was significantly lower than that of model group, and higher than that of normal group. The CYP2E1 expressed in endochylema of liver cells among gland ＇alveolus region around central veins of hepatic lobules. The positive rate of expression of CYP2E1 was 70%, while CYP2E1 almost was not be found expressed in normal group. The positive rate of expression of treatment group treated with compound Ganzheng being （20%） was significantly lower than that of model group. There were no significant dif- ference compared with that of normal group. Conclusion： Compound ganzheng has the roles of anti - lipid peroxide and getting rid of oxygen free radicals.