益肾降浊颗粒质量标准研究

目的建立益肾降浊颗粒的质量标准。方法采用薄层色谱法对处方中大黄、黄芪进行定性鉴别；采用高效液相色谱法对样品中大黄素和大黄酚进行含量测定。结果定性鉴别方法专属性强．阴性对照无干扰；大黄素在2．45～39．2μg·mL^-1浓度范围内．大黄酚在2．76～44．16μg·mL^-1浓度范围内，浓度与峰面积线性关系良好，大黄素的平均回收率为99．53％．RSD为0．78％；大黄酚平均回收率为97．28％,RSD为0．69％。结论该方法简便、准确可靠，可用于该制剂的质量控制。

Study on quality standard for Yishenjiangzhuo granules

OBJECTIVE To establish a quality standard for Yishenjiangzhuo granules. METHODS Rhubarb and Radix Astragali were identified by the TLC;Emodin and chrysophanol in Yishenjiangzhuo granules were determined by HPLC. RESULTS Qualitation identification was highly specific, and the blank test showed no interference;Emodin showed a good linear relationship at a range between 2.45 and 39.2μg·mL^-1, the average recovery was 99.53 %, and RSD was 0.78 % ; chrysophano. showed a good linear relationship at a range between 2.76 and 44.16μg·mL^-1, the average recovery was 97.28 %, and RSD was 0.69 % . CONCLUSION The method is simple, accurate and can be used for quality control of Yishenjiangzhuo granules.