鼻咽癌特征性microRNA表达谱分析

目的采用microRNA(miRNA)基因芯片的方法分析在鼻咽癌组织中特征性表达的miRNA,寻找鼻咽癌的分子肿瘤学标志物。方法分别选取8例鼻咽非角化型癌和8例鼻咽部慢性炎性组织,Trizol法提取总RNA,与含有人723条miRNAs探针的基因芯片进行杂交,检测8例鼻咽癌组织和鼻咽部慢性炎性组织中miRNA表达谱,筛选差异表达的miRNA。结果 miRNA表达谱分析发现在鼻咽癌差异表达的miRNA共31个,其中上调6个,下调25个。结论通过基因芯片的方法发现了在鼻咽癌中31个差异表达的miRNA,为进一步探索鼻咽癌的发生、发展及早期诊断奠定了基础。

Characteristic miRNAs expression profile of nasopharyngeal carcinoma

Objective To study the expression profiles of microRNA（miRNA） in nasopharyngeal carcinoma by microarray technique and explore the potential molecular signature specific for nasopharyngeal carcinoma.Methods Tissues were obtained from 8 cases of nasopharyngeal non-keratinizing carcinoma and 8 cases of chronic nasopharyngeal inflammation.Total RNA was extracted by Trizol method and hybridized with human 723 miRNA microarrays.The miRNA expression profiles of nasopharyngeal carcinoma and chronic inflammation was analyzed and differential miRNA expression between nasopharyngeal carcinomas and chronic inflammation was determined.Results miRNA expression profiling was performed to screen the miRNA expression change of the nasopharyngeal carcinoma.Thirty-one miRNAs were identified to be differentially expressed,in which 6 were overexpressed and 25 were underexpressed in nasopharyngeal carcinoma.Conclusion Thirty-one differentially expressed miRNAs were detected in nasopharyngeal carcinoma by microarray method.The study of the differentially expressed miRNAs in nasopharyngeal carcinoma may play a foundation for the advanced studies to explore the carcinogenesis,development and early diagnosis of the nasopharyngeal carcinoma.