阿霉素耐药性研究与流式细胞分析

目的 探讨流式细胞术在阿霉素多药耐药研究中的特殊意义。方法 对两组不同耐药倍数的阿霉素耐药细胞系（S－180R和BGC－823／DOX）进行动态、对比性流式细胞分析。结果 流式细胞术在高倍数耐药细胞系S－180R中表现出与亲代细胞耐药性的直观的整体差异，在低倍数耐药细胞系BGC－823／DOX中则可以定量地分辨与亲代细胞耐药性的细微差异。在动态分析中，检测到耐药细胞群中个体细胞耐药性的变化，即个体细胞内荧光含量的接近，细胞荧光峰的集中。结论 流式细胞术在阿霉素耐药性研究中具有灵敏、准确、定量的作用。

The analysis of multi-drug resistance of doxorubicin with flow cytometry

Objective To evaluate the significance of flow cytometry (FCM) in the analysis of multi drug resistance of doxorubicin. Methods The level of doxorubicin or Rh 123 in S 180R and BGC 823/DOX, which were two cell lines with different drug resistance, was measured by FCM comparatively and continuously. Results The total fluorescence profile peaks of S 180R, a high resistant cell line, were predominantly different from those of S 180, the parent cell line, by the FCM analysis. The subtle fluorescence profile differences between BGC 823/DOX (a low resistant cell line) and the parent cell line were quantitatively measured on the FCM map. The changes of each resistant cell fluorescence from the S 180R cells could be displayed by continuous tests, the more near the fluorescence level of each cell was, the more the fluorescence pike of whole cells centralized. Conclusion FCM is a sensitive, accurate and quantitative test in the analysis of doxorubicin drug resistance.