大鼠缺锌时股骨骺生长板的病理形态学改变

为观察大鼠缺锌时股骨骺生长板的病理形态学改变 ,将 30只Wistar大鼠按体重随机分为 3组 :缺锌组 ,对照组和对喂组 ,每组动物 1 0只。缺锌组和对照组饲料锌含量分别为 3 9和 2 4 7mg kg,对喂组饲对照组饲料 ,饲料摄入量与缺锌组相同。动物喂养 8周后处死 ,在普通光学显微镜下观察大鼠股骨组织病理变化并运用形态计量学方法定量描述 ,测定血清骨钙素和生长激素含量。结果发现缺锌组大鼠股骨骺生长板软骨细胞畸形、数量减少 ,骺端骨小梁纤细、疏松、排列紊乱、髓腔相对扩大 ,骨小梁体积显著减少 ,骨小梁板密度降低 ,骨小梁间隙增大 ,血清骨钙素和生长激素含量减少。提示缺锌一方面通过降低循环中生长激素水平抑制骺软骨细胞增殖分化 ,从而使软骨内骨化障碍 ,骨骼生长迟缓 ;另一方面缺锌抑制成骨细胞活性 ,破坏成骨细胞和破骨细胞之间的动态平衡 ,导致骨骼骨量生成减少 ,骨重塑和骨再建不良 ,骨空间结构紊乱。

Zinc deficiency on pathological changes of femur epiphyseal growth plate in rats

To study zinc deficiency on pathological changes of femur epiphyseal growth plate in rats thirty Wistar rats were randomly divided into three groups: zinc deficient group (Zd), control group (Ctr), and pair fed group (Zp) to study the effects of zinc deficiency on femur epiphyseal growth plate of rats and the mechanism involved. After 8 weeks feeding, the histomorphology of right femur indicated that chondrocytes in the epiphyseal growth plate of Zd group were generally ill organized and misshaped. The number of chondrocytes decreased. Trabecular bone in the epiphyseal of Zd group were also ill organized, scarce and slim. The cavities of marrow in Zd animals were significantly larger than those of Ctr and Zp. The volume of trabecular bone, the mean trabecular plate density of Zd rats were significantly decreased, but their mean trabecular plate space was significantly increased. In addition, the Zd animals had significantly lower concentration of osteocalcin and growth hormone in sera. The above results suggested that zinc deficiency impare proliferation and differentiation of chondrocytes and balance between osteoblast and osteoclast function by reduce growth hormone levels in sera. The lack of zinc in diet resulted in disorder of bone molding and ultrastructure.