Mechanisms of the CYNAP phenomenon: evidence in the Bw49/Bw50 model for epitopes with different spatial orientation of antibody |
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| Authors: | D M Lublin F C Grumet |
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| Affiliation: | Department of Pathology, Stanford University School of Medicine, California, USA |
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| Abstract: | The mechanism of the cytotoxic-negative, absorption-positive (CYNAP) phenomenon was studied using the model of the Bw49/Bw50 split of the BW21 antigen. Anti-Bw49 antibody bound 60% as well to Bw 50 as to Bw49 cells; however, even at a cytotoxic titer of 64 against Bw49 cells, the antibody was not cytotoxic to Bw50 Cells. At equal numbers of antibody molecules bound, the anti-Bw49 antibody activated C4 and C3, and induced lysis for Bw49 but not for the Bw50 cells. These data are consistent with a model in which different spatial orientations for shared epitopes can account for CYNAP reactivity for at least some selected Bw4/Bw6-associated splits of B locus antigens. |
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| Keywords: | C1 C2…, complement components C-FDA carboxyfluorescein diacetate CYNAP cytotoxic-negative, absorption-positive EGTA ethylenebis(oxyethylenenitrilo)-tetraacetic a acid RT room temparature SEM standard of error of the mean VBS veronal buffered saline |
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