糖尿病鼠肺间质免疫组织化学的研究

目的 探讨糖尿病鼠肺间质免疫组织化学的研究。方法 建立实验性糖尿病模型后四周，采用特殊染色法，免疫组织化学法和MD20型彩色图像分析系统对特殊染色与免疫组化学染色都先进行图像测量，修正和分析，测定积分光密度和相对含量，并同对照组进行比较。结果 糖尿病早期肺间质弹性纤维和网状纤维增多，增粗，相对含量增高。结论 糖尿病早期肺间质病变主要表现为细胞外基质增加，其机制为高糖状态导致非酶糖化等作用增强。这是导致弥漫性肺间质纤维化的病理生理基础。

THE STUDY ON CHANGES OF IMMUNOHISTOCHEMISTRY IN PULMONARY INTERSTITUM OF DIABETIC RATS

Objective To evaluate the changes of extra cellular matrix in diabetic rats lungs. Methods After build up the experimental model of diabetes for four weeks, use special staining immunohistochemical method and image analysis, the changes of distribution, area, integral optical density and relative contents of lung ECM in diabetic rats were studied. Results The distributions of elastic fibres, collagen fibres and reticular fibres increased and roughened. The type IV collagen strong staining was localized in alveolar septum,the basement membranes of bronchiole and capillary, and distributed in cordal ,plexiform .Laminin was strongly stained and found in alveolar septum, epithelial cells of alveolar ,and the basement membranes of bronchiole and pulmonary vessels in cordal and linear distribution. The image analysis showed the area,integral optical density and relative contents of elastic fibres, collagen fibres, reticular fibres, type IV collagen and laminin were larger in diabetic rats lungs than in control groups. Conclusion These results showed that the changes of extracellular matrix in early diabetic rats lungs were abnormal.