留兰香油主要成分香芹酮和柠檬烯的GC含量测定及薄层鉴别

目的:建立留兰香油中的主要有效成分的测定方法。方法:采用Aglient6890N气相色谱仪,HP-5弹性石英毛细管柱(30 m×0.323 mm×0.25μm),氢火焰离子化检测器,气化室温度250℃,检测室温度280℃,进样分流比为20∶1,程序升温。采用正十一烷为内标对照品溶液。采用薄层色谱法对其主要成分进行定性鉴别。结果:留兰香油中柠檬烯质量浓度在41.7¹042.5μg·mL-1(R=0.999)间,香芹酮质量浓度在102.81042.5μg·mL-1(R=0.999)间,香芹酮质量浓度在102.8²570μg·mL-1(R=0.999)间均呈良好的线性关系,平均回收率分别为101.7%,103.0%,RSD值为1.92%和1.59%。薄层色谱清晰,空白对照无干扰。结论:含量测定方法简便,准确,稳定性高,能有效测定留兰香油中有效成分的含量。薄层鉴别专属性强,可用于留兰香油的定性鉴别。

Determination of Main Components of Spearmint Oil by GC and Identification by TLC

Objective：To establish the determination method of main active components of spearmint oil. Methods： Aglient 6890N gas chromatograph was used with HP -5 elastic quartz capillary column （30 m×0.323mm×0.25μm） and hydrogen flame ionization detector. The gasification chamber temperature was 250 ℃ ; testing room temperature was 280 ℃ ; sample split ratio was 20 ： 1 ; temperature program control was used. N - hexane was selected as internal standard solution. TLC was used to identify the main active components of spearmint oil. Results ： For the GC quantitation method, the calibration curve of limonene of spearmint oil displayed ideal linearity in the range of 41.7 - 1042.5 μg mL-l. And the calibration curve of carvone displayed ideal linearity in the range of 102.8 - 2570 μg mL-1. The average recoveries were 101.7% , 103% and RSD values were 1.92% and 1.59%. The TLC figure was clear. The blank test showed no in- terference. Conclusion：The quantitative determination method was sensitive, reliable and repeatable, and could effectively detect the effective components of spearmint oil. TLC was specific and has less interference.