不同经穴TEAS对低血压大鼠痛阈及血压调节效应的实验研究

目的:观察不同经穴经皮穴位电刺激(transcutaneous electrical acupoint stimulation,TEAS)对低血压大鼠痛阈及血压的调节效应。方法:将50只低血压模型大鼠随机分成足三里、三阴交、太冲、曲池和合谷穴5个组,每组10只,采用经皮穴位电刺激法介入治疗,刺激参数为疏密波(频率2/100Hz)、强度(4±1)mA,时间为40 min,每天治疗1次,连续治疗3 d。采用辐射热甩尾法测量造模后、治疗中及治疗后不同时段痛阈。采用无创动物血压观察系统BP-5测量TEAS治疗前、治疗疗程结束后即时、治疗后不同时段的大鼠尾动脉收缩压。结果:5个经穴组经皮电刺激后均能不同程度提高大鼠痛阈,治疗后与自身痛阈相比具有统计学差异(P<0.05),除合谷穴外,4个经穴组经皮电刺激后均能不同程度提高低血压大鼠血压,治疗后与治疗前血压相比具有统计学差异(P<0.05)。结论:足三里、三阴交、太冲、曲池、合谷穴均能提高低血压大鼠痛阈,以足三里穴提高幅度最明显,具有较好的镇痛效应,同时,足三里、三阴交、太冲、曲池穴均能提高低血压大鼠血压,且以足三里穴升压效应最为明显。

Research on Pain Threshold and Blood Pressure Regulating Effect of TEAS at Different Acupoints on Hypotensive Rats

Objective ： To observe the regulating effect of TEAS on pain threshold and blood pressure at different acu- points of hypotensive model rats. Method：The 50 hypotensive model rats were randomly divided into Zusanli group, Sany- injiao group,Taichong group, Quchi group and Hegu group（ n = 10）. TEAS intervention treatment was carried out to the rats of different acupoint groups, with stimulus parameter for dilatational wave （frequency 2/100Hz）, strength for degree of local muscle tremoring （4 ＋ 1 ） mA, time for 40 min, once a day, for 3 days continuously. The pain threshold was meas- ured by radiant heat - induced tail flick test in different time points ： after model establishment, during treatment and dif- ferent time periods after treatment. Meanwhile,the caudal artery systolic blood pressure of rats was observed by noninva- sive animal blood pressure observation system BP- 5 at different time points：before treatment and different time periods after treatment. Result：After TEAS intervention treatment, the pain thresholds of rats all rose to varying degrees in each acupoint groups. There was a statistical difference comparing one＇ s own pain threshold with that after treatment （P 〈 0.05 ）. The blood pressure of rats rose to varying degrees after TEAS treatment in four acupoint groups except Hegu group. There was a statistical difference comparing one＇ s own blood pressure with that after treatment （P 〈 O. 05 ）. Con- clusion： Zusanli,Sanyinjiao,Taichong,Quchi and Hegu all can rise pain threshold of hypotensive rats. And Zusanli was most significant in the rising range with great analgesia effect. Meanwhile, Zusanli, Sanyinjiao,Taichong and Quchi can in- crease the blood pressure of hypotensive rats, and Zusanli with the most significant increasing effect.