卡铂复合液诱导胃癌细胞凋亡和p53蛋白表达的研究

作者比较了卡铂液和卡铂复合液对胃癌细胞株（ＮＫＭ－２５）细胞凋亡和ｐ５３蛋白表达的影响，研究表明随着卡铂液作用时间的延长，胃癌细胞凋亡数量和ｐ５３蛋白表达明显增加，免疫组织化学研究亦显示卡铂液作用后可以使胃癌细胞ｐ５３蛋白的表达增强。卡铂复合液对胃癌细胞凋亡数量和ｐ５３蛋白表达的影响与卡铂液的作用相比未见显著性差异（Ｐ＞０．０５）。研究提示：①卡铂液诱导胃癌细胞凋亡与ｐ５３蛋白的表达密切相关，可能为ｐ５３蛋白依赖性；②卡铂复合液保持了卡铂液所具有的对胃癌细胞凋亡的诱导作用。结合作者的前期研究，提示采用卡铂复合液腹腔内化疗，腹腔粘连和腹膜纤维化程度明显减轻，并发症少，是治疗胃肠道癌肿等的一种安全、有效的区域化疗方法。

Study on Apoptosis and p53 Protein Expression Induced by Carboplatin Mixture Liquid in Gastric Cancer Cell Line

The apoptosis and expression of p53 protein in gastric carcinoma cell line(NKM 25) induced by carboplatin mixture liquid were studied and compared with the effect of carboplatin liquid. The results showed that the percentage of apoptosis and expression of p53 protein were increased significantly as extention of the treatment with carboplatin liquid. Comparing the effect of carboplatin liquid on amount of apoptosis with that of carboplatin mixture liquid, there was no significant differeance between them( P >0.05). The data suggested that there was a close relationship between expression of p53 and apoptosis of NEK 25 cell line, and the carboplatin liquid induced apoptosis might be in p53 dependent pathways. Furthermore, it seems that the carboplayin mixture liquid still remained the same cytotoxic effects on the gastric cancer as the carboplatin liquid. The liquid fits the intraperitoneal chemotherapy for gastric malignacies more safely and effectively after surgical resection, because it can obviously reduce abdominal adhesion, peritoneal fibrosis and complication.