男性无精子症、少精子症患者Y染色体AZF微缺失筛查

目的为在福州地区建立原发无精子症、严重少精子症Y染色体AZF区域微缺失分子诊断方法.方法根据Y染色体长臂无精子因子区(AZF)序列标签位点(sequence taggedsites,STS)和短臂SRY基因等共设计10对引物,采用多重聚合酶链反应-琼脂糖凝胶电泳方法对95例原发无精子症、42例少精子症、20例精子活动力＞50%男性患者及50例正常生育男性外周血DNA进行Y染色体AZF区域微缺失分析.结果95例原发性无精子症患者检出13例AZF区域STS位点缺失,占13.7%,42例严重少精子症患者检出AZF区域STS位点缺失5例,占11.9%,正常生育男性、精子活动力＞50%患者无一例AZF微缺失.结论Y染色体AZF区域微缺失与男性无精子症、严重少精子症密切相关.

Y-chromosome AZF microdeletion for male infertility

Objective: To establishe gene diagnosis assey of Y - chromosome AZF microdeletion for male azoospemria or oligozoospermia in Fuzhou. Methods: Using 10 Tag Sequence Site(STS) primers binding method and multiplex PCR with agarose gel elec-trophoresis were performed to detect AZF microdletions in 95 patients azoospermia and 42 patients with oligozoospermia . Results: 13 AZF microdeletion were conformed 95 patients azoospennia (13.7 %) and 5 with 42 patients with oligozoospermia (11.9%). No AZF microdeletion was form in 50 patients normal male and 20 patients male where active rate of sperm were less then 50% . Conclusions: Y-chromosome AZF microdeletion was associated with male azoospennia or oligozoospermia.