AGEs与葡萄糖浓度对血管内皮细胞增生的影响

为研究非酶促糖化终末产物(AGEs)和不同浓度葡萄糖对血管内皮细胞增生的影响,用氚标记胸腺嘧啶(~3H-TdR)掺入法,测定不同浓度葡萄糖、AGEs以及抗氧化剂(维生素E)对血管内皮细胞增生的影响。结果:11mmol/L葡萄糖加AGEs(5 mg/L)、30 mmol/L葡萄糖、11 mmol/L葡萄糖+AGEs+维生素E组的血管内皮细胞的2 h~3H-TdR掺入量分别为对照组的4.69倍(P<0.01)、2.15倍(P<0.05)和1.46倍(P<0.05)。提示:高糖和AGEs均能直接刺激血管内皮细胞增生,高糖及AGEs同时存在时更为明显,而抗氧化剂维生素E则抑制内皮细胞增生。

Effect of AGEs and Concentration of Glucose on Proliferation of Vascular Endothelial Cells Cultured in Vitro

The objective was to study the effect of AGEs on proliferation of endothelial cells of cord vein from newborn. 3H-TdR incoporation rate of cultured cells was used as parameter for cell proliferation and VitE affected proliferation of endothelial cells. After two hours of endothelial cells of cord vein from newborn, 11 mmol/L glucose, AGEs (5 mg/L), VitE and 30 mmol/L glucose were cultured to test 3H-TdR incoporation rate. The 3H-TdR incorporation rates were 4.69 times (P<0.01), 2.15 times (P< 0.05) and 1.46 times (P<0.05) higher than the control group respectively. High glucose and AGEs stimulate proliferation of endothelial cells. Especially, high glucose and AGEs are in cultured cells. At same time VitE could inhibit proliferation of endothelial cells.