| 缺血预处理对兔缺血再灌注心肌bcl—2，bax，p53基因表达的影响 |
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| 摘 要： |
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| 关 键 词： | 缺血预处理 兔 缺血再灌注 心肌 bcl－2 bax p53 基因表达 影响 细胞凋亡 |
Effect of ischemic preconditioning on myocardial bcl-2, bax, p53 gene expression during ischemia/reperfusion period in rabbits] |
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| Jun-mei Xu,Rong Tan,Dong-xu Hu. Effect of ischemic preconditioning on myocardial bcl-2, bax, p53 gene expression during ischemia/reperfusion period in rabbits][J]. Bulletin of Hunan Medical University, 2003, 28(2): 111-113 |
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| Authors: | Jun-mei Xu Rong Tan Dong-xu Hu |
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| Affiliation: | Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China. |
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| Abstract: | OBJECTIVE: To investigate the effects of ischemic preconditioning on myocardial bcl-2, bax, p53 gene expression during ischemia/reperfusion period in rabbits. METHODS: Twenty four rabbits were randomly allocated to three groups (n = 8), pseudo-operation group(Group P), ischemia/reperfusion group (Group IR) and ischemic preconditioning group(Group IP). Group IR and Group IP were subjected to three hours of left anterior descending coronary artery occlusion followed for three hours of reperfusion. Ischemic preconditioning was achieved by three 5-minute cycles of ischemia, each followed by 5 minutes of reperfusion. Apoptosis index(AI) and protein expression of Bcl-2, Bax, and p53 in the border zone of myocardium of ischemic area at risk were obtained with a flow cytometry. RESULTS: Compared with Group IR, AI was significantly reduced in Group IP(P < 0.01). Bcl-2 expressions was higher in Group IP than in Group IR, while bax and p53 expressions were lower in Group IP than in Group IR. CONCLUSION: The rabbit myocardial apoptosis induced by ischemic preconditioning inhibited ischemia-reperfusion in vivo partly related to the modulating of bcl-2, bax and p53 expression. |
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