| hTR基因反义核酸对K562细胞端粒酶活性和凋亡的影响 |
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| 引用本文： | 胡杰英,杨胜利,蒋东霞. hTR基因反义核酸对K562细胞端粒酶活性和凋亡的影响[J]. 癌变．畸变．突变, 2002, 14(3): 151-154. DOI: 10.3969/j.issn.1004-616X.2002.03.005 |
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| 作者姓名： | 胡杰英 杨胜利 蒋东霞 |
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| 作者单位： | 河南省肿瘤研究所医学生物室,河南,郑州,450003 |
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| 基金项目： | 河南省科技攻关项目;001170204; |
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| 摘 要： | 目的: 研究人类端粒酶(hTR)基因的反义核酸对K562细胞端粒酶活性和细胞凋亡的影响.方法:采用TRAP法检测K562细胞在反义核酸处理前后端粒酶活性的变化,以及采用TUNEL法观察反义核酸处理前后细胞凋亡的变化.结果:hTR基因反义核酸能有效抑制K562细胞的端粒酶活性,并且诱导K562细胞发生凋亡.结论:hTR基因反义核酸能显著抑制肿瘤细胞的端粒酶活性,为恶性肿瘤治疗提供了一条新的有效的途径.
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| 关 键 词： | 端粒酶 K562细胞: 反义治疗: 凋亡 |
| 文章编号： | 1004-616X(2002)03-0151-03 |
| 修稿时间： | 2001-10-22 |
EFFECT OF ANTISENSE OLIGONUCLEOTIDE AGAINST hTR RNA ON THE TELOMERASE ACTIVITY AND APOPTOSIS IN K562 CELLS |
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| Abstract: | Purpose: To study the inhibitory effect of human telomerase activity and the apoptosis by an antisense oligonucleotide against human telomerase RNA(hTR) in K562 cells. Methods: Telomerase activity of K562 cells with antisense treatment was measured using the TRAP assay, and apoptosis was studied by means of TUNEL method. Results: The telomerase activity was effectively inhibited, and the apoptosis was induced by the antisense treatment. Conclusion: Antisense oligonucleotide against hTR can effectively inhibit the telo {merase} activity of K562 cells. |
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| Keywords: | telomerase K562 cell antisense treatment apoptosis |
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