灯盏花素冰片注射液对实验性脑缺血的保护作用

目的:研究灯盏花素冰片注射液(SBI)对大鼠局灶性脑缺血和小鼠缺血缺氧性损伤的保护作用。方法:采用大鼠急性不完全性脑缺血实验,以大脑皮层含水量为指标初步筛选组方剂量。采用大鼠局灶性脑缺血再灌注损伤模型(MCAO)对模型大鼠进行神经症状和行为学评分,测定大脑皮层含水量,计算脑梗死区百分比,组织病理切片观察海马损伤程度,并测定脑组织内总抗氧化能力(T-AOC),丙二醛(MDA)和乳酸(LD)含量以及超氧化物歧化酶(SOD)、肌酸激酶(CK)、乳酸脱氢酶(LDH)、Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶、总ATP酶(T-ATP)活力等生化指标;采用小鼠急性不完全性脑缺血实验和小鼠常压耐缺氧实验,测定其存活时及对耐缺氧能力的影响。结果:灯盏花素12mg/kg与冰片0.48mg/kg配伍对模型大鼠降低其脑含水量作用最为显著。与模型对照组比较,SBI组尾静脉注射能使动物的神经行为学评分,脑梗死率和脑含水量显著降低,能显著改善海马区神经元的形态,同时还能明显增强MCAO后大鼠脑组织内T-AOC能力,增加脑组织SOD,显著减少脑组织内MDA含量,同时对于脑组织内CK活力、LDH活力也有显著的增加作用,还能显著减少MCAO后大鼠脑组织LD含量,显著增加Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶及T-ATP酶活力。结论:SBI高剂量组可以极显著延长急性不完全性脑缺血小鼠存活时间和耐缺氧能力。结论:SBI对实验性脑缺血具有保护作用。

Protective effects of scutellarin and borneolum injection on experimental cerebral ischemia in animals

Aim:To study the protective effects of scutellarin and borneolum injection(SBI) on the focal cerebral ischemia-reperfusion injury in rats and anoxia in mice.Methods:Acute brain ischemia in rats was created in the section of the optimal compatible doses of SBI.The rats and mice were administered with SBI or nimodipine injection via vena caudalis before the operation.The middle cerebral artery occlusion(MCAO) model in rats according to the modified suture-occluded method of Zea Longa was made to measure the neurological deficit score,cerebral edema and infarction area.The neurocytological morphosis of hippocampus was observed according to the pathological section.Some biochemical indicators such as the activity of creatine kinase(CK),superoxide dismutase(SOD),lactic dehydrogenase(LDH) and ATPase were measured.And the content of CK,MDA and LDH were also measured.At the same time,acute brain ischemia in mice was produced by bilateral ligation of carotid arteries with vagus nerves.Acute anoxia in mice was produced by hypoxia under normal pressure.Results:The optimal compatible dose of SBI consisting of scutellarin(12 mg/kg) and borneolum(0.48 mg/kg) was established.Compared with to the MCAO rats,the SBI groups markedly decreased neurological deficit score,the percentage of brain water,cerebral edema and infarction area,while significantly reducing activities of LDH,CK and content of LD,enhancing activity of SOD and T-AOC and declining the content of MDA in brain.SBI significantly prolonged the survival time of hypoxia mice and mice in the acute brain ischemia.Conclusion:SBI has the protective effects on experimental cerebral ischenic in animals.