Investigation of Nakano lens proteins |
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| Authors: | Debdutta Roy Margaret H Garner Abraham Spector Deborah Carper Paul Russell |
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| Institution: | 1. Biochemistry and Molecular Biology Laboratory, Department of Ophthalmology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, U.S.A.;2. Laboratory of Vision Research, National Eye Institute, National Institute of Health, U.S. Department of Health, Education and Welfare, Bethesda, Maryland 20014, U.S.A. |
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| Abstract: | Changes in the protein chemistry of the Nakano lens with age and developing cataract and comparison with normal mouse lens protein are reported. It was found that significant differences exist between the protein of the normal and the cataractous lens. In Nakano lenses high molecular weight disulfide-linked aggregates, disulfide-linked cytosol polypeptides to the fiber membrane, an apparent increase in the concentration of degraded polypeptides, disulfide crosslinking of low molecular weight species and marked differences in membrane polypeptide profiles were observed. A striking similarity was found between these observations with the Nakano cataract and previous reports of the changes in protein chemistry in the development of senile human cataract. It can be concluded that although the initiating event for induction of the cataract may differ, the sequence of events following such insult may be similar. |
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| Keywords: | lens cataract membrane genetic immunochemistry |
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