| 正交实验法优选鱼腥草胰蛋白酶抑制剂的提取工艺 |
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| 引用本文： | 杜旭东,刘同祥,张宗申,牛建昭,王继峰. 正交实验法优选鱼腥草胰蛋白酶抑制剂的提取工艺[J]. 时珍国医国药, 2007, 18(10): 2337-2338 |
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| 作者姓名： | 杜旭东 刘同祥 张宗申 牛建昭 王继峰 |
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| 作者单位： | 1. 大连工业大学生物与食品工程学院,辽宁,大连,116034
2. 北京中医药大学基础医学院,北京,100029 |
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| 基金项目： | 国家自然科学基金项目（No.30130220No.30371775） |
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| 摘 要： | 目的研究鱼腥草中胰蛋白酶抑制剂活性成分的最佳提取条件。方法以提取液对胰蛋白酶的抑制率为指标,采用正交试验法对提取温度(A)、溶剂用量(B)、pH值(C)、提取时间(D)4个因素进行优选研究,并以优选出的提取方案进行验证实验。结果因素B对提取效果有极显著的影响,因素A、C均有显著的影响,因素D影响不显著。结论鱼腥草中胰蛋白酶抑制活性成分有效部位的最佳提取工艺为A1B2C3D2。
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| 关 键 词： | 鱼腥草 胰蛋白酶抑制剂 正交实验 |
| 文章编号： | 1008-0805（2007）10-2337-01 |
| 收稿时间： | 2007-03-13 |
| 修稿时间： | 2007-05-15 |
Optimize the Extraction Procedure of Trypsin Inhibitor of Houttuynia cordata Thunb. by Orthogonal Design |
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| DU Xu-dong,LIU Tong-xiang,ZHANG Zong-shen,NIU Jian-zhao,WANG Ji-feng. Optimize the Extraction Procedure of Trypsin Inhibitor of Houttuynia cordata Thunb. by Orthogonal Design[J]. Lishizhen Medicine and Materia Medica Research, 2007, 18(10): 2337-2338 |
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| Authors: | DU Xu-dong LIU Tong-xiang ZHANG Zong-shen NIU Jian-zhao WANG Ji-feng |
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| Affiliation: | 1. College of Biology and Food Technology, Dalian Polytechnic University, Dalian 116034 , China; 2. School of Basic Medical Sciences, Beijing University of Chinese Medicine, Bering, 100029, China |
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| Abstract: | Objective To optimize the extraction procedure for trypsin inhibitor from Houttuynia cordata Thunb..Methods The inhibition rate of trypsin activity was used as screening parameter,we employed the orthogonal design method to test the effects of four factors,including temperature of extraction solution(A),volume of extraction solution(B),pH value of extraction solution(C) and time of extraction(D),on the extraction of trypsin inhibitor.Results Factor B performed the most significant effect on the extraction,and factors A and C came the next and factor D the last.Conclusion The optimum extraction technology is A1B2C3D2 when the inhibition ratio of trypsin activity is considered as preferential parameter. |
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| Keywords: | Houttuynia cordata Thunb. Trypsin inhibitor Orthogonal design |
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