缺氧和高糖对大鼠肾成纤维细胞ICAM-1mRNA表达的影响

目的:探讨缺氧和高糖对肾成纤维细胞(NRK)细胞间粘附分子-1(ICAM-1)mRNA表达的影响。方法:将大鼠NRK分6组进行体外培养:(1)正常浓度葡萄糖组(常糖组):5.6mmol/L葡萄糖;(2)常糖+缺氧组:5.6mmol/L的葡萄糖+100μmol/L的CoCl2;(3)高糖A组:15mmol/L葡萄糖;(4)高糖+缺氧A组:15mmol/L葡萄糖+100μmol/L的CoCl2;(5)高糖B组:30mmol/L葡萄糖;(6)高糖+缺氧B组:30mmol/L葡萄糖+100μmol/L的CoCl2。分别于24h、48h取各组NRK采用RT-PCR检测ICAM-1mRNA表达水平。结果:与常糖组相比,高糖A组,高糖B组ICAM-1mRNA表达量逐渐升高(P<0.01),常糖+缺氧组、高糖+缺氧A、B组ICAM-1mRNA表达量也升高(P<0.01);高糖+缺氧A、B组ICAM-1mRNA表达量分别比高糖A、B组明显升高(P<0.01);高糖A、B组和常糖+缺氧组、高糖+缺氧A、B组48hICAM-1mRNA表达量均高于24h表达量(P<0.01)。结论:高糖和缺氧均可导致ICAM-1mRNA高表达,缺氧可能进一步增加高糖上调ICAM-1的表达。

The Effect of Hypoxia on ICAM-1 of Rat Kidney Fibroblasts Exposed to High Glucose

Objective:To explore the effect of hypoxia and high glucose on ICAM-1 of rat kidney fibroblasts.Method:NRK cells were divided into six groups,and then cultured in vitro:(1)normal glucose group:5.6mmol/L glucose;(2)normal glucose+hypoxia group:5.6mmol/L glucose with 100μmol/L CoCl2;(3)high glucose A group:15mmol/L glucose;(4)high glucose+hypoxia A group:15mmol/L glucose+100μmol/L CoCl2;(5)high glucose B group:30mmol/L glucose;(6)high glucose+hypoxia B group:30mmol/L glucose+100μmol/L CoCl2.The expression of ICAM-1 mRNA was measured by RT-PCR after 24h,48h,respectively.Results:Compared with normal glucose group,the ICAM-1 mRNA of high glucose A and B group was remarkably increased(P<0.01),the ICAM-1 mRNA was remarkably increased in normal glucose+hypoxia group,high glucose+hypoxia group A and B(P<0.01);compared with high glucose A and B group,the ICAM-1 mRNA was remarkably increased in high glucose+hypoxia group A and B(P<0.01);compared with 24h groups,the ICAM-1 mRNA was remarkabley increased in 48h groups.Conclusion:High glocose is able to upregulate the expression of ICAM-1 mRNA.Hypoxia is able to upregulate the expression of ICAM-1 mRNA under the high glucose environment.